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https://hdl.handle.net/2440/43540
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Type: | Journal article |
Title: | Relaxin signalling in THP-1 cells uses a novel phosphotyrosine-dependent pathway |
Author: | Anand Ivell, R. Heng, K. Bartsch, O. Ivell, R. |
Citation: | Molecular and Cellular Endocrinology, 2007; 272(1-2):1-13 |
Publisher: | Elsevier Sci Ireland Ltd |
Issue Date: | 2007 |
ISSN: | 0303-7207 1872-8057 |
Statement of Responsibility: | Ravinder Anand-Ivell, Kee Heng, Olaf Bartsch and Richard Ivell |
Abstract: | The heterodimeric peptide hormone relaxin acts through the novel G-protein coupled receptor LGR7 to elicit the production of cAMP in the human monocyte cell line THP-1. The very small number of receptors on the cell surface, and the lack of response in cell membranes imply the involvement of a cytoplasmic signal amplification process. Here we show that this process comprises a novel and specific tyrosine kinase activity close to the receptor, and involves neither protein kinase A, mitogen-activated protein kinase, nor phosphoinositide-3 kinase activities as major upstream components. Furthermore, this novel involvement of a tyrosine kinase activity is cell-type dependent, being largely absent from LGR7-transfected HEK293T cells, and receptor-dependent; vasoactive intestinal peptide or isoproterenol signalling in the same cells does not require this tyrosine kinase activity. |
Keywords: | Cell Line Humans Relaxin Cyclic AMP-Dependent Protein Kinases Extracellular Signal-Regulated MAP Kinases Phosphotyrosine Membrane Proteins Receptors, G-Protein-Coupled Receptors, Peptide Cyclic AMP Protein Kinase Inhibitors Transfection Signal Transduction Organ Specificity Phosphorylation Phosphatidylinositol 3-Kinases |
Description: | Copyright © 2007 Elsevier Ireland Ltd All rights reserved. |
DOI: | 10.1016/j.mce.2007.04.001 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/506028/description#description |
Published version: | http://dx.doi.org/10.1016/j.mce.2007.04.001 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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