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https://hdl.handle.net/2440/44183
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Type: | Journal article |
Title: | The pneumococcal two-component signal transduction system RR/HK06 regulates CbpA and PspA by two distinct mechanisms |
Author: | Standish, A. Stroeher, U. Paton, J. |
Citation: | Journal of Bacteriology, 2007; 189(15):5591-5600 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2007 |
ISSN: | 0021-9193 1098-5530 |
Statement of Responsibility: | Alistair J. Standish, Uwe H. Stroeher, and James C. Paton |
Abstract: | We have previously shown that CbpA, a major pneumococcal virulence factor, is regulated by the two-component signal transduction system RR/HK06 (A. J. Standish, U. H. Stroeher, and J. C. Paton, Proc. Natl. Acad. Sci. USA 102:7701-7706, 2005). However, additional unidentified regulated factors appeared to be responsible for differences in adherence and the ability of Streptococcus pneumoniae to cause disease in a mouse model. Here, we identified a number of other regulated genes by overexpressing the system. cbpA, along with a cotranscribed upstream gene, showed substantial increases in expression when RR06 was overexpressed in S. pneumoniae strains D39 and TIGR4. However, there were no other similarities between these strains. In D39, rr06 overexpression decreased expression of numerous factors, including the major virulence factor gene pspA. Further investigation of cbpA regulation by RR/HK06, using mutants with mutations in both HK06 and RR06, suggested that rather than the norm, cbpA transcription was activated when RR06 was in the nonphosphorylated form. Although other factors, such as pspA and gls24, are regulated by this system, these genes appear to be repressed when RR06 is in its phosphorylated form. |
Keywords: | Streptococcus pneumoniae Protein Kinases Bacterial Proteins RNA, Bacterial RNA, Messenger Electrophoresis, Polyacrylamide Gel Signal Transduction Gene Expression Regulation, Bacterial Mutation Histidine Kinase |
Description: | Copyright © 2007, American Society for Microbiology. All Rights Reserved. |
DOI: | 10.1128/JB.00335-07 |
Published version: | http://jb.asm.org/cgi/content/abstract/189/15/5591 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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