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https://hdl.handle.net/2440/44324
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Type: | Journal article |
Title: | Regulation of the CYP27B1 5 '-flanking region by transforming growth factor-beta in ROS 17/2.8 osteoblast-like cells |
Author: | Turner, A. Dwivedi, P. May, B. Morris, H. |
Citation: | The Journal of Steroid Biochemistry and Molecular Biology, 2007; 103(3-5 Sp Iss):322-325 |
Part of: | 13th Workshop on Vitamin D (Victoria, British Columbia, Canada, April 2006) / edited by R. Bouillon, A.W. Norman and J.R. Pasqualini |
Publisher: | Pergamon-Elsevier Science Ltd |
Issue Date: | 2007 |
ISSN: | 0960-0760 1879-1220 |
Statement of Responsibility: | Andrew G. Turner, Prem P. Dwivedi, Brian K. May and Howard A. Morris |
Abstract: | The biologically active form of vitamin D, 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), regulates osteoblast proliferation and differentiation. Production of 1,25(OH)(2)D(3) is catalysed by the enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP27B1). Though highly expressed in the kidney, the CYP27B1 gene is also expressed in non-renal tissues including bone. It is hypothesised that local production of 1,25(OH)(2)D(3) by osteoblasts plays an autocrine or paracrine role. The aim of this study was to investigate what factors regulate expression of the CYP27B1 gene in osteoblast cells. ROS 17/2.8 osteoblast cells were transiently transfected with plasmid constructs containing the 5'-flanking sequence of the human CYP27B1 gene fused to a luciferase reporter gene. Cells were treated with either parathyroid hormone (PTH), 1,25(OH)(2)D(3), transforming growth factor-beta (TGF-beta) or insulin-like growth factor-1 (IGF-1) and luciferase activity was measured 24h later. The results showed that 1,25(OH)(2)D(3) did not alter expression of the reporter construct, however treatment with PTH, IGF-1 and TGF-beta decreased expression by 18, 53 and 58% respectively. The repressive action of TGF-beta was isolated to the region between -531 and -305bp. These data suggest that expression of the 5'-flanking region for the CYP27B1 gene in osteoblast cells may be regulated differently to that previously described in kidney cells. |
Keywords: | Cell Line, Tumor Osteoblasts Animals Humans Rats 25-Hydroxyvitamin D3 1-alpha-Hydroxylase Transforming Growth Factor beta Gene Expression Regulation, Enzymologic 5' Flanking Region |
Description: | Crown copyright © 2006 Published by Elsevier Ltd. |
DOI: | 10.1016/j.jsbmb.2006.12.041 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/333/description#description |
Published version: | http://dx.doi.org/10.1016/j.jsbmb.2006.12.041 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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