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https://hdl.handle.net/2440/46605
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Type: | Journal article |
Title: | Direct stimulation of osteoclastogenesis by MIP-1alpha: evidence obtained from studies using RAW264 cell clone highly responsive to RANKL |
Author: | Watanabe, T. Kukita, T. Kukita, A. Wada, N. Toh, K. Nagata, K. Nomiyama, H. Iijima, T. |
Citation: | Journal of Endocrinology, 2004; 180(1):193-201 |
Publisher: | Soc Endocrinology |
Issue Date: | 2004 |
ISSN: | 0022-0795 1479-6805 |
Statement of Responsibility: | T Watanabe, T Kukita, A Kukita, N Wada, K Toh, K Nagata, H Nomiyama and T Iijima |
Abstract: | <jats:p>Macrophage inflammatory protein-1alpha (MIP-1alpha) is a member of the CC chemokines. We have previously reported the use of a whole bone marrow culture system to show that MIP-1alpha stimulates the formation of osteoclast-like multinucleated cells. Here we use rat bone marrow cells deprived of stromal cells, and clones obtained from murine macrophage-like cell line RAW264 to show that MIP-1alpha acts directly on cells in osteoclast lineage. We obtained several types of RAW264 cell clones, one of these clones, designated as RAW264 cell D clone (D clone), showed an extremely high response to receptor activator of NFkappaB ligand (RANKL) and tumor necrosis factor-alpha (TNF-alpha), while the other clone, RAW264 cell N clone (N clone), demonstrated no response to RANKL or TNF-alpha. Although both clones expressed receptor activator NFkappaB (RANK) before being stimulated for differentiation, only the D clone expressed cathepsin K when cells were stimulated to differentiate to osteoclasts. MIP-1alpha stimulated the formation of mononuclear preosteoclast-like cells from rat bone marrow cells deprived of stromal cells. MIP-1alpha also stimulated formation of osteoclast-like multinucleated cells from the D clone, when these cells were stimulated with RANKL and TNF-alpha. These findings provide strong evidence to show that MIP-1alpha acts directly on cells in the osteoclast lineage to stimulate osteoclastogenesis. Furthermore, pretreatment of RAW264 cell D clone with MIP-1alpha significantly induced adhesion properties of these cells to primary osteoblasts, suggesting a crucial role for MIP-1alpha in the regulation of the interaction between osteoclast precursors and osteoblasts in osteoclastogenesis.</jats:p> |
Description: | Copyright © 2004 by Society for Endocrinology |
DOI: | 10.1677/joe.0.1800193 |
Published version: | http://joe.endocrinology-journals.org/cgi/reprint/180/1/193 |
Appears in Collections: | Aurora harvest 6 Dentistry publications |
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