Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/46670
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Fibroblastic cells from human periapical granulation tissue preferentially form calcified matrices in decalcified boiled rat bone |
Author: | Maeda, H. Wada, N. Fujii, S. Akamine, A. |
Citation: | Cell and Tissue Research, 2005; 320(1):135-140 |
Publisher: | Springer |
Issue Date: | 2005 |
ISSN: | 0302-766X 1432-0878 |
Statement of Responsibility: | Hidefumi Maeda, Naohisa Wada, Shinsuke Fujii and Akifumi Akamine |
Abstract: | We have been studying the potential of human fibroblastic cells (HFC) from periapical granulation tissue to form a calcified matrix. Recently, we reported that inflamed periapical granulation tissue contains osteogenic cells. In the present study, we tested the hypothesis that HFC, cultured with decalcified bone (DB) of rat, might form much greater calcified matrices than with rat decalcified boiled bone (DBB), which was originally prepared as a negative control. HFC were cultured with DB or DBB in the presence or absence of 2 mM β-glycerophosphate (β-GP) and 50 μg/ml ascorbic acid. After six weeks of culture, a number of von Kossa-positive globular structures were unexpectedly observed inside DBB, but not DB. Without HFC, such structures were never seen in DBB incubated with 2 mM β-GP and 50 μg/ml ascorbic acid. DB cultured with HFC under the same conditions did not show these structures. Electron-microscopic observation revealed that matrix vesicles aggregated on collagen fibrils around globular structures in DBB. Energy dispersive X-ray microanalysis confirmed that these structures were calcified matrices composed of calcium and phosphate. These results suggest that human periapical granulation tissue contains cells responsible for the formation of calcified matrices in DBB, and that DBB could serve as an excellent scaffold for the calcification of HFC, rather than DB. |
Keywords: | Periapical granulation tissue Decalcified bone Decalcified and boiled bone Calcified matrices Human Rat (WKA) |
Description: | The original publication can be found at www.springerlink.com |
DOI: | 10.1007/s00441-004-1052-x |
Published version: | http://dx.doi.org/10.1007/s00441-004-1052-x |
Appears in Collections: | Aurora harvest Dentistry publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.