Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/47285
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Baculovirus as Mammalian cell expression vector for gene therapy: an emerging strategy |
Author: | Ghosh, Supid Parvez, Md Khalid Banerjee, Kakoli Sarin, Shiv K. Hasnain, Seyed E. |
Citation: | Molecular Therapy, 2002; 6(1):5-11 |
Publisher: | Academic Press - Elsevier |
Issue Date: | 2002 |
ISSN: | 1525-0016 |
School/Discipline: | School of Molecular and Biomedical Science |
Abstract: | The monopoly of insect cells to host baculovirus Autographa califomica multiple nuclear polyhedrosis virus (AcMNPV) as a eukaryotic gene expression system has been shattered with the growing evidence that it also infects mammalian cells in culture. Although AcMNPV fails to replicate in vertebrate cells, it does express alien genes with levels of expression that are dependent on the strength of the promoter used to drive transcription of the foreign gene. It also has been reported that the recombinant AcMNPV enters human hepatic cells in culture preferentially and specifically in comparison with the other mammalian cells of different origin and sources. This has resulted in the use of AcMNPV as a potent mammalian cell delivery system as a xenovector for gene therapy, more precisely liver-specific gene delivery in vitro and in vivo. |
Keywords: | baculovirus; human gene therapy; AcMNPV; xenovector |
Description: | © 2008 American Society of Gene Therapy |
DOI: | 10.1006/mthe.2000.0643 |
Appears in Collections: | Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.