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https://hdl.handle.net/2440/47429
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Type: | Journal article |
Title: | ABO genotyping by PCR-RFLP and cloning and sequencing |
Author: | Haak, W. Burger, J. Alt, K. |
Citation: | Anthropologischer Anzeiger: Bericht uber die biologisch-anthropologische Literatur, 2004; 62(4):397-410 |
Publisher: | E. Schweizerbart'sche Verlagsbuchhandlung |
Issue Date: | 2004 |
ISSN: | 0003-5548 2363-7099 |
Abstract: | A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications. Four fragments of exon 6 and 7 of the ABO gene were amplified and digested by in total 7 restriction endonucleases. Particular attention was paid to the base changes at nucleotide positions 261(delG), 297, 526, 703, 721, 771, 796 and 1060(delC) in order to distinguish the six common alleles A101, A201, B, O01, O02 and O03. Furthermore, this method also enables determination of seven of the less frequent alleles: A104, A204, Ax02, Ax03, O05, O06 and O07. The method was applied successfully to a series of blood samples with known phenotypes and genotypes as well as DNA extracted from a thirty year old blood stain and an ancient tooth sample. However, working with ancient DNA requires additional cloning and sequencing of the RFLP-typing results due to DNA post mortem damages such as deaminations, which could lead to false typing results. |
Keywords: | Tooth Humans Chromosome Deletion Postmortem Changes DNA Transposable Elements ABO Blood-Group System Reproducibility of Results Cloning, Molecular Polymerase Chain Reaction Sequence Analysis, DNA Forensic Medicine Phenotype Polymorphism, Restriction Fragment Length Alleles Archaeology Blood Stains History, Ancient |
DOI: | 10.1127/anthranz/62/2004/397 |
Published version: | http://dx.doi.org/10.1127/anthranz/62/2004/397 |
Appears in Collections: | Aurora harvest 6 Earth and Environmental Sciences publications Environment Institute publications |
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