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https://hdl.handle.net/2440/48110
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DC Field | Value | Language |
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dc.contributor.author | Shen, Z. | - |
dc.contributor.author | Crotti, T. | - |
dc.contributor.author | Flannery, M. | - |
dc.contributor.author | Matsuzaki, K. | - |
dc.contributor.author | Goldring, S. | - |
dc.contributor.author | McHugh, K. | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Biochimica et Biophysica Acta: international journal of biochemistry and biophysics, 2007; 1769(11-12):659-667 | - |
dc.identifier.issn | 0006-3002 | - |
dc.identifier.issn | 1878-2434 | - |
dc.identifier.uri | http://hdl.handle.net/2440/48110 | - |
dc.description.abstract | The calcitonin receptor (CTR) is expressed in a wide variety of tissues and cell types. In bone, its expression is restricted to osteoclasts, the cells that mediate bone resorption. The human CTR (hCTR) gene has a complex structural organization that exhibits similarity to the porcine (pCTR) and mouse (mCTR) CTR genes. In these species, alternative splicing of a single gene generates multiple CTR isoforms that are distributed in both tissue-specific and species-specific patterns. However, the structural organization of the 5' putative regulatory region and transcriptional mechanisms responsible for tissue-specific expression of the different CTR isoforms are not fully defined. The present studies were undertaken to characterize the structural organization of the 5'-region of the hCTR and identify the regulatory regions involved in osteoclast-specific transcriptional activation. Analysis of mRNA prepared from human osteoclasts using reverse transcription-polymerase chain reaction (RT-PCR) and transient transfection of hCTR promoter-luciferase reporter constructs identified two regions in the 5'-flanking sequence of the hCTR gene that regulated CTR gene expression in osteoclasts. Both of these putative promoters were responsive to the osteoclast-inducing cytokine, receptor activator of NF-kappaB ligand (RANKL) and demonstrated trans-activation by the RANKL-induced transcription factor nuclear factor of activated T cells (NFATc1), consistent with a role in regulating CTR gene expression in osteoclasts. | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Science | - |
dc.source.uri | http://dx.doi.org/10.1016/j.bbaexp.2007.08.005 | - |
dc.subject | Cells, Cultured | - |
dc.subject | Osteoclasts | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Receptors, Calcitonin | - |
dc.subject | RNA, Messenger | - |
dc.subject | Transfection | - |
dc.subject | Gene Expression Regulation | - |
dc.subject | Tissue Distribution | - |
dc.subject | RANK Ligand | - |
dc.subject | Promoter Regions, Genetic | - |
dc.title | A novel promoter regulates calcitonin receptor gene expression in human osteoclasts | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.bbaexp.2007.08.005 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Crotti, T. [0000-0002-5422-3758] | - |
Appears in Collections: | Aurora harvest 6 Pathology publications |
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