The Arabidopsis Ca²⁺ / H⁺ exchangers, AtCAX1 and AtCAX3, are shown by co-localisation, interaction and complementation to participate in plant Ca²⁺ homeostasis.

Abstract

This research was performed over 10 months as part of a Masters in Biotechnology (Plant Biotechnology). The literature review was previously assessed but has been modified somewhat to address small changes in the research focus of the project. Although the research manuscript contained herein will provide the first draft of a future publication in Plant Cell and Physiology Journal, due to time constraints, additional data relevant to that publication may be collected. Additional data that has been collected which was not conclusive or integral to the focus of the research manuscript is provided within the appendices. The research manuscript details investigations into the interactions of Arabidopsis calcium transporters, while the appendices contain additional information pertaining to protoplast transformations, plasmid construction and plant media recipes.