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https://hdl.handle.net/2440/50774
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Type: | Journal article |
Title: | FAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate |
Author: | Eyre, N. Cleland, L. Mayrhofer, G. |
Citation: | Biochemical and Biophysical Research Communications, 2008; 370(3):404-409 |
Publisher: | Academic Press Inc |
Issue Date: | 2008 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Nicholas S. Eyrea, Leslie G. Clelandb and Graham Mayrhofer |
Abstract: | Fatty acid translocase (FAT/CD36) is one of several proteins implicated in receptor-mediated uptake of long-chain fatty acids (LCFAs). We have tested whether levels of FAT/CD36 correlate with cellular oleic acid import, using a Tet-Off inducible transfected CHO cell line. Consistent with our previous findings, FAT/CD36 was enriched in lipid raft-derived detergent-resistant membranes (DRMs) that also contained caveolin-1, the marker protein of caveolae. Furthermore in transfected cells, plasma membrane FAT/CD36 co-localized extensively with the lipid raft-enriched ganglioside GM1, and partially with a caveolin-1-EGFP fusion protein. Nevertheless, even at high levels of expression, FAT/CD36 did not affect uptake of oleic acid. We propose that the ability of FAT/CD36 to mediate enhanced uptake of LCFAs is dependent on co-expression of other proteins or factors that are lacking in CHO cells. |
Keywords: | FAT/CD36 Long-chain fatty acid transport Lipid rafts Caveolae Tet-off |
Description: | Copyright © 2008 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V. |
DOI: | 10.1016/j.bbrc.2008.02.164 |
Published version: | http://dx.doi.org/10.1016/j.bbrc.2008.02.164 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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