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https://hdl.handle.net/2440/51141
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Type: | Journal article |
Title: | Viability of Listeria monocytogenes in co-culture with Acanthamoeba spp. |
Author: | Akya, A. Pointon, A. Thomas, C. |
Citation: | FEMS Microbiology Ecology, 2009; 70(1):20-29 |
Publisher: | Elsevier Science BV |
Issue Date: | 2009 |
ISSN: | 0168-6496 1574-6941 |
Statement of Responsibility: | Alisha Akya, Andrew Pointon and Connor Thomas |
Abstract: | Listeria monocytogenes is a human pathogen, ubiquitous in the environment, and can grow and survive under a wide range of environmental conditions. It contaminates foods via raw materials or food-processing environments. However, the current knowledge of its ecology and, in particular, the mode of environmental survival and transmission of this intracellular pathogen remains limited. Research has shown that several intracellular pathogens are able to survive or replicate within free-living amoebae. To examine the viability of L. monocytogenes in interaction with Acanthamoeba spp., bacteria were co-cultured with three freshly isolated amoebae, namely Acanthamoeba polyphaga, Acanthamoeba castellanii and Acanthamoeba lenticulata. The survival of bacteria and amoebae was determined using culture techniques and microscopy. Under the experimental conditions used, all amoebae were able to eliminate bacteria irrespective of the hly gene. Bacteria did not survive or replicate within amoeba cells. However, extra-amoebic bacteria grew saprophytically on materials released from amoebae, which may play an important role in the survival of bacteria under extreme environmental conditions. |
Keywords: | Listeria monocytogenes Acanthamoeba interaction |
Description: | © 2008 Federation of European Microbiological Societies/Blackwell Publishing Ltd |
DOI: | 10.1111/j.1574-6941.2009.00736.x |
Published version: | http://dx.doi.org/10.1111/j.1574-6941.2009.00736.x |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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