Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/51311
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Van Bergen, N. | - |
dc.contributor.author | Wood, J. | - |
dc.contributor.author | Chidlow, G. | - |
dc.contributor.author | Trounce, I. | - |
dc.contributor.author | Casson, R. | - |
dc.contributor.author | Ju, W. | - |
dc.contributor.author | Weinreb, R. | - |
dc.contributor.author | Crowston, J. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Investigative Ophthalmology and Visual Science, 2009; 50(9):4267-4272 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.issn | 1552-5783 | - |
dc.identifier.uri | http://hdl.handle.net/2440/51311 | - |
dc.description.abstract | <h4>Purpose</h4>The transformed RGC-5 retinal ganglion cell line is used widely in glaucoma research. Increased resistance to glutamate was noted in published literature and led to the recharacterization of the RGC-5 cell line.<h4>Methods</h4>Characterization of the RGC-5 cell line was performed by sequencing of a region of the nuclear Thy1 gene and mitochondrial DNA sequencing of a region of the d-loop and tRNA(Phe) gene. Marker expression was examined in undifferentiated cells, and cells differentiated with 50 microg/mL succinyl concanavalin A (S Con A) for 3 days. Glutamate sensitivity was examined in undifferentiated and S Con A differentiated cells by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 24-hours of glutamate treatment.<h4>Results</h4>RGC-5 cells were found to be of mouse (Mus musculus), not rat (Rattus norvegicus), origin by mitochondrial and nuclear DNA analyses. RGC-5 DNA sequenced in a second laboratory was subsequently found to be of M. musculus origin. Cells stained positively for the neuronal markers beta-tubulin and PGP9.5 and for the microtubule-associated protein tau, but not for known markers of ganglion cells such as neurofilaments or Thy1.2, suggesting that they likely represented a lineage of mouse neuronal precursor cells. Differentiation with S Con A did not increase RGC-5 sensitivity to glutamate excitotoxicity or increase the expression of retinal or ganglion cell marker proteins.<h4>Conclusions</h4>Investigators using cells designated as RGC-5 should confirm the species to be of rat origin and retinal-specific marker expression before considering their use as retinal ganglion-like cells. | - |
dc.description.statementofresponsibility | Nicole J. Van Bergen, John P. M. Wood, Glyn Chidlow, Ian A. Trounce, Robert J. Casson, Won-Kyu Ju, Robert N. Weinreb, and Jonathan G. Crowston | - |
dc.language.iso | en | - |
dc.publisher | Assoc Research Vision Ophthalmology Inc | - |
dc.source.uri | http://dx.doi.org/10.1167/iovs.09-3484 | - |
dc.subject | Retinal Ganglion Cells | - |
dc.subject | Cell Line, Transformed | - |
dc.subject | Animals | - |
dc.subject | Mice | - |
dc.subject | Rats | - |
dc.subject | Glutamic Acid | - |
dc.subject | Concanavalin A | - |
dc.subject | Fluorescent Antibody Technique, Indirect | - |
dc.subject | Cell Differentiation | - |
dc.subject | Biomarkers | - |
dc.title | Recharacterization of the RGC-5 retinal ganglion cell line | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1167/iovs.09-3484 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Chidlow, G. [0000-0001-7371-0239] | - |
dc.identifier.orcid | Casson, R. [0000-0003-2822-4076] | - |
Appears in Collections: | Aurora harvest Opthalmology & Visual Sciences publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.