Adelaide Research & Scholarship
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https://hdl.handle.net/2440/51618
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| Type: | Journal article |
| Title: | Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates |
| Author: | Brotherton, P. Endicott, P. Beaumont, M. Barnett, R. Austin, J. Cooper, A. Sanchez, J. |
| Citation: | Forensic Science International: Genetics Supplement Series, 2008; 1(1):19-21 |
| Publisher: | Elsevier |
| Issue Date: | 2008 |
| ISSN: | 1875-1768 1875-175X |
| Statement of Responsibility: | Paul Brotherton, Phillip Endicott, Mark Beaumont, Ross Barnett, Jeremy Austin, Alan Cooper, Juan J. Sanchez |
| Abstract: | High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus. © 2008 Elsevier Ireland Ltd. All rights reserved. |
| Keywords: | DNA damage Genotyping Low copy number DNA SNPs |
| DOI: | 10.1016/j.fsigss.2007.10.111 |
| Published version: | http://dx.doi.org/10.1016/j.fsigss.2007.10.111 |
| Appears in Collections: | Aurora harvest Earth and Environmental Sciences publications Environment Institute Leaders publications |
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