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https://hdl.handle.net/2440/52241
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Type: | Journal article |
Title: | Co-expression of CYP27B1 enzyme with the 1.5 kb CYP27B1 promoter-luciferase transgene in the mouse |
Author: | Anderson, P. Hendrix, I. Sawyer, R. Zarrinkalam, R. Manavis, J. Sarvestani, G. May, B. Morris, H. |
Citation: | Molecular and Cellular Endocrinology, 2008; 285(1-2):1-9 |
Publisher: | Elsevier Sci Ireland Ltd |
Issue Date: | 2008 |
ISSN: | 0303-7207 1872-8057 |
Statement of Responsibility: | Paul H. Anderson, Ivanka Hendrix, Rebecca K. Sawyer, Reza Zarrinkalam, Jim Manavis, Ghafar T. Sarvestani, Brian K. May and Howard A. Morris |
Abstract: | The renal enzyme 25-hydroxyvitamin D 1alpha-hydroxylase (CYP27B1), responsible for the synthesis of circulating. 1,25-dihydroxyvitamin D (1,25D), is also expressed in a number of non-renal tissues. The regulation of CYP27B1 expression by the short flanking promoter outside the kidney is, however, largely unknown. We have used a transgenic mice expressing the 1.5kb promoter of the human CYP27B1 gene fused to the firefly luciferase gene in order to investigate tissue-specific CYP27B1 expression. These transgenic animals demonstrated co-localised luciferase and endogenous CYP27B1 expression in kidney proximal convoluted tubular cells. Strong co-expression of luciferase and CYP27B1 also occurred in neurons and Purkinje cells of the cerebellum and in Leydig and Sertoli cells of the testes. Other tissues to exhibit CYP27B1-promoter directed luciferase activity included lung, prostate, trabecular bone and jejunum as well as the choroid epithelium. The tissue specific changes in luciferase activity were age-related. These findings demonstrate that the proximal 1.5kb 5' flanking region of the CYP27B1 gene directs the expression of CYP27B1 in a number of known and novel tissues in a specific manner. |
Keywords: | Animals Mice, Transgenic Humans Mice 25-Hydroxyvitamin D3 1-alpha-Hydroxylase Recombinant Fusion Proteins Gene Expression Regulation, Enzymologic Tissue Distribution Transgenes 5' Flanking Region Male Promoter Regions, Genetic |
Description: | Crown copyright © 2008 Published by Elsevier Ireland Ltd. |
DOI: | 10.1016/j.mce.2007.12.018 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/506028/description#description |
Published version: | http://dx.doi.org/10.1016/j.mce.2007.12.018 |
Appears in Collections: | Aurora harvest 5 Earth and Environmental Sciences publications |
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