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https://hdl.handle.net/2440/52580
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Type: | Journal article |
Title: | Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein |
Author: | May, K. Morona, R. |
Citation: | Journal of Bacteriology, 2008; 190(13):4666-4676 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2008 |
ISSN: | 0021-9193 1098-5530 |
Statement of Responsibility: | Kerrie L. May and Renato Morona |
Abstract: | The IcsA (VirG) protein of Shigella flexneri is a polarly localized, outer membrane protein that is essential for virulence. Within host cells, IcsA activates the host actin regulatory protein, neural Wiskott-Aldrich syndrome protein (N-WASP), which in turn recruits the Arp2/3 complex, which nucleates host actin to form F-actin comet tails and initiate bacterial motility. Linker insertion mutagenesis was undertaken to randomly introduce 5-amino-acid in-frame insertions within IcsA. Forty-seven linker insertion mutants were isolated and expressed in S. flexneri Delta icsA strains. Mutants were characterized for IcsA protein production, cell surface expression and localization, intercellular spreading, F-actin comet tail formation, and N-WASP recruitment. Using this approach, we have identified a putative autochaperone region required for IcsA biogenesis, and our data suggest an additional region, not previously identified, is required for N-WASP recruitment. |
Keywords: | Hela Cells Humans Shigella flexneri Actins Trypsin Bacterial Proteins Bacterial Outer Membrane Proteins DNA-Binding Proteins Transcription Factors Blotting, Western Mutagenesis, Insertional Wiskott-Aldrich Syndrome Protein |
DOI: | 10.1128/JB.00093-08 |
Published version: | http://dx.doi.org/10.1128/jb.00093-08 |
Appears in Collections: | Aurora harvest 5 Microbiology and Immunology publications |
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