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https://hdl.handle.net/2440/53462
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Type: | Journal article |
Title: | Hyaluronidase 3 (HYAL3) knockout mice do not display evidence of hyaluronan accumulation |
Author: | Atmuri, V. Martin, D. Hemming, R. Gutsol, A. Byers, S. Sahebjam, S. Thilveris, J. Mort, J. Carmona, E. Anderson, J. Dakshinamurti, S. Triggs-Raine, B. |
Citation: | Matrix Biology, 2008; 27(8):653-660 |
Publisher: | Elsevier Science BV |
Issue Date: | 2008 |
ISSN: | 0945-053X 1569-1802 |
Statement of Responsibility: | Vasantha Atmuri, Dianna C. Martin, Richard Hemming, Alex Gutsol, Sharon Byers, Solmaz Sahebjam, James A. Thliveris, John S. Mort, Euridice Carmona, Judy E. Anderson, Shyamala Dakshinamurti, and Barbara Triggs-Raine |
Abstract: | Hyaluronidases are endoglycosidases that initiate the breakdown of hyaluronan (HA), an abundant component of the vertebrate extracellular matrix. In humans, six paralogous genes encoding hyaluronidase-like sequences have been identified on human chromosomes 3p21.3 (HYAL2–HYAL1–HYAL3) and 7q31.3 (SPAM1–HYAL4–HYALP1). Mutations in one of these genes, HYAL1, were reported in a patient with mucopolysaccharidosis (MPS) IX. Despite the broad distribution of HA, the HYAL1-deficient patient exhibited a mild phenotype, suggesting other hyaluronidase family members contribute to constitutive HA degradation. Hyal3 knockout (Hyal3−/−) mice were generated to determine if HYAL3 had a role in constitutive HA degradation. Hyal3−/− mice were viable, fertile, and exhibited no gross phenotypic changes. X-ray analysis, histological studies of joints, whole-body weights, organ weights and the serum HA levels of Hyal3−/− mice were normal. No evidence of glycosaminoglycan accumulation, including vacuolization, was identified in the Hyal3−/− tissues analyzed. Remarkably, the only difference identified in Hyal3−/− mice was a subtle change in the alveolar structure and extracellular matrix thickness in lung-tissue sections at 12–14 months-of-age. We conclude that HYAL3 does not play a major role in constitutive HA degradation. |
Keywords: | Hyaluronan Hyaluronidase Hyal3 Mucopolysaccharidoses Glycosaminoglycan |
DOI: | 10.1016/j.matbio.2008.07.006 |
Published version: | http://dx.doi.org/10.1016/j.matbio.2008.07.006 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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