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https://hdl.handle.net/2440/55112
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Type: | Journal article |
Title: | Anti-genotoxic effects of tea catechins against reactive oxygen species in human lymphoblastoid cells |
Author: | Sugisawa, A. Kimura, M. Fenech, M. Umegaki, K. |
Citation: | Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 2004; 559(1-2):97-103 |
Publisher: | Elsevier Science BV |
Issue Date: | 2004 |
ISSN: | 1383-5718 1879-3592 |
Statement of Responsibility: | Ayako Sugisawa, Michiyo Kimura, Michael Fenech and Keizo Umegaki |
Abstract: | Using the cytokinesis-block micronucleus assay in WIL2-NS cells, we investigated the effects of six tea constituents, (−)-epigallocatechin-3-O-gallate (EGCg), (−)-epicatechin-3-O-gallate (ECg), (−)-epigallocatechin (EGC), (−)-epicatechin (EC), (+)-catechin (+C) and gallic acid (GA), on chromosomal damage in two ways; induction by each component on its own and prevention against treatment of reactive oxygen species (ROS). None of the tea constituents induced chromosomal damage at <10μM. On the other hand, EGCg, EGC, ECg, +C and GA prevented H₂O₂-induced chromosomal damage in a dose-dependent manner with a significant effect detected at 10 μM. Chromosomal damage induced by tert-butylhydroperoxide was apparently prevented by EGCg and ECg at 0.3 μM, but not by EGC and GA even at 10 μM, suggesting that the galloyl group linked to flavan-3-ol is needed for the observed protective effect. These results suggest that physiological concentration of tea constituents are not genotoxic but rather anti-genotoxic against ROS, although their preventive effects are slightly different depending on their chemical structure. |
Keywords: | Tea catechins Chromosomal damage Micronucleus assay WIL2-NS cells Reactive oxygen species |
DOI: | 10.1016/j.mrgentox.2004.01.002 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/522820/description#description |
Published version: | http://dx.doi.org/10.1016/j.mrgentox.2004.01.002 |
Appears in Collections: | Aurora harvest 5 Pharmacology publications |
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