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https://hdl.handle.net/2440/55258
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Type: | Journal article |
Title: | Axonal transport of BDNF precursor in primary sensory neurons |
Author: | Wang, H. Linyan Wu, L. Song, X. Luo, X. Zhong, J. Rush, R. Zhou, X. |
Citation: | European Journal of Neuroscience, 2006; 24(9):2444-2452 |
Publisher: | Blackwell Science Ltd |
Issue Date: | 2006 |
ISSN: | 0953-816X 1460-9568 |
Statement of Responsibility: | Hui Wang, Linda Lin-yan Wu, Xing-Yun Song, Xue-Gang Luo, Jin-Hua Zhong, Robert A. Rush and Xin-Fu Zhou |
Abstract: | Recent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague–Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions. |
Keywords: | Spinal Cord Neurons, Afferent Sciatic Nerve Spinal Nerve Roots Animals Mice Rats Rats, Sprague-Dawley Brain-Derived Neurotrophic Factor Blotting, Western Nerve Crush Axonal Transport |
DOI: | 10.1111/j.1460-9568.2006.05138.x |
Published version: | http://dx.doi.org/10.1111/j.1460-9568.2006.05138.x |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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