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https://hdl.handle.net/2440/55690
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dc.contributor.author | Hall, C. | - |
dc.contributor.author | Reichel, M. | - |
dc.contributor.author | Ellis, J. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Veterinary Parasitology, 2006; 140(1-2):61-68 | - |
dc.identifier.issn | 0304-4017 | - |
dc.identifier.issn | 1873-2550 | - |
dc.identifier.uri | http://hdl.handle.net/2440/55690 | - |
dc.description | Copyright © 2006 Elsevier B.V. All rights reserved. | - |
dc.description.abstract | Aim: To determine the performance characteristics of two enzyme-linked immunosorbent assays (ELISAs) manufactured by Institut Pourquier (IP) for the detection of antibodies against Neospora caninum in bovine sera. Methods: Sera from 526 cattle were assayed in two ELISAs (IP) for the detection of anti-N. caninum antibodies. Results from a further ELISA (IDEXX) were used to provide the "gold standard" N. caninum infection status of the cattle and the ELISA results assessed by two-graph receiver operating characteristic (TG-ROC) analysis. Results: TG-ROC analysis suggested changes to one of the IP ELISA protocols, arriving at a cut-off threshold that was different to the one recommended by the manufacturer. With that change, both of the ELISAs performed with high sensitivity and specificity (in excess of 98%) for bovine sera. Conclusions: The analysis of the two IP ELISAs when used on individual bovine sera demonstrated high sensitivity and specificity. TG-ROC analyses optimised the cut-off point suggested by the manufacturer for one of these commercial diagnostic assays and found agreement with the manufacturer's cut-off regarding the other assay. This will help with the accurate identification of infected animals and thereby contributing to the control of neosporosis. © 2006 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | C.A. Hall, M.P. Reichel, J.T. Ellis | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Science BV | - |
dc.source.uri | http://dx.doi.org/10.1016/j.vetpar.2006.03.016 | - |
dc.subject | Neospora caninum | - |
dc.subject | Protozoa | - |
dc.subject | Cattle | - |
dc.subject | Serology | - |
dc.subject | ELISA | - |
dc.subject | ROC analysis | - |
dc.title | Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.vetpar.2006.03.016 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
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