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https://hdl.handle.net/2440/5645
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Type: | Journal article |
Title: | Anti-inflammatory activity of a lipid fraction (lyprinol) from the NZ green-lipped mussel |
Author: | Whitehouse, M. Macrides, T. Kalafatis, N. Betts, W. Haynes, D. Broadbent, J. |
Citation: | Inflammopharmacology: experimental and clinical studies, 1997; 5(3):237-246 |
Publisher: | Springer Science and Business Media LLC |
Issue Date: | 1997 |
ISSN: | 0925-4692 1568-5608 |
Abstract: | A lipid-rich extract, preparared by supercritical fluid extraction of fresh stabilized mussel powder (Lyprinol), showed significant anti- inflammatory (AI) activity given therapeutically and prophylactically po to Wistar and Dark Agouti rats developing either (a) adjuvant-induced polyarthritis or (b) collagen(II)-induced autoallergic arthritis, with ED50≤ 15 mg/kg; c.f. naproxen ≤ 25 mg/kg or various therapeutic oils (flaxseed, evening primrose, fish) ≤ 1800 mg/kg given orally. Lyprinol showed little or no activity in acute irritation assays (carrageenan, kaolin, histamine) indicating it is not mimicking rapid-acting NSAIDs. Incorporating Lyprinol into arthritigenic adjuvants composed of heat-killed Mycobacterium tuberculosis suspended in olive oil or squalane, effectively prevented arthritis development at a dose of 5 mg/rat. By contrast, 'dummy adjuvants' prepared with Mycobacterium tuberculosis and flaxseed, evening primrose or fish oils were still arthritigenic in Dark Agouti rats (doses of oil = 90 mg/rat). Lyprinol subfractions inhibited leukotriene-B4 biosynthesis by stimulated human polymorphonuclear leukocytes in vitro, and prostaglandin- E2 production by activated human macrophages in vitro. Much of this AI activity was associated with polyunsaturated fatty acids and natural antoxidants (carotenoids, etc.). In contrast to NSAIDs, Lyprinol is non- gastrotoxic in disease-stressed rats at 300 mg/kg po and does not seem to affect platelet aggregation (human, rat). These data show Lyprinol to be a reproducible, relatively stable, source of bioactive lipids with much greater potency than plant/marine oils currently used as nutritional supplements to ameliorate signs of inflammation. |
DOI: | 10.1007/s10787-997-0002-0 |
Published version: | http://dx.doi.org/10.1007/s10787-997-0002-0 |
Appears in Collections: | Aurora harvest 5 Pathology publications |
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