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https://hdl.handle.net/2440/56472
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dc.contributor.author | Bilusich, D. | - |
dc.contributor.author | Jackway, R. | - |
dc.contributor.author | Musgrave, I. | - |
dc.contributor.author | Tyler, M. | - |
dc.contributor.author | Bowie, J. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Rapid Communications in Mass Spectrometry, 2009; 23(17):2628-2636 | - |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.issn | 1097-0231 | - |
dc.identifier.uri | http://hdl.handle.net/2440/56472 | - |
dc.description | Copyright © 2009 John Wiley & Sons, Ltd. | - |
dc.description.abstract | Positive and negative ion electrospray mass spectrometry together with Edman sequencing (when appropriate) has been used to sequence the host-defence peptides secreted from skin glands of the tree frog Litoria peronii. The peptide profiles are different in winter and summer. In winter, the frog produces small amounts of the known caerin 1.1 [GLLSVLGSVAKHVLPHVVPVIAEHL-NH(2)] (a wide-spectrum antibiotic) and caerin 2.1 [GLVSSIGRALGGLLADVVKSKQPA-OH], a narrow-spectrum antibiotic and an inhibitor of neuronal nitric oxide synthase. The major peptides produced throughout the year are the pGlu-containing peroniins 1.1 to 1.5 (e.g. peroniin 1.1 [pEPWLPFG-NH(2)], a smooth muscle contractor from 10(-7) M), and caerulein [pEQDY(SO(3)H)TGWMDF-NH(2)], a known and potent smooth muscle contractor from 10(-10) M. There are also some precursors to the peroniin 1 peptides, only detected in the skin secretion in summer, which are inactive and appear to be all (or part) of the spacer peroniin 1 peptides, e.g. peroniin 1.1b [SEEEKRQPWLPFG-NH(2)]. There are three members of the Litoria peronii Group of tree frogs classified in Australia, namely, L. peronii, L. rothii and L.tyleri. A comparison of the skin peptide profiles of L. peronii with those reported previously for L. rothii suggests that either these two species of tree frog are not as closely related as determined previously on morphological grounds, or that skin peptide divergence in tree frogs of this Group is more extensive than in others that have been studied. | - |
dc.description.statementofresponsibility | Daniel Bilusich, Rebecca J. Jackway, Ian F. Musgrave, Michael J. Tyler, and John H. Bowie | - |
dc.language.iso | en | - |
dc.publisher | John Wiley & Sons Ltd | - |
dc.source.uri | http://dx.doi.org/10.1002/rcm.4164 | - |
dc.subject | Skin | - |
dc.subject | Animals | - |
dc.subject | Anura | - |
dc.subject | Bacteria | - |
dc.subject | Antimicrobial Cationic Peptides | - |
dc.subject | Amphibian Proteins | - |
dc.subject | Anti-Bacterial Agents | - |
dc.subject | Spectrometry, Mass, Electrospray Ionization | - |
dc.subject | Seasons | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Molecular Sequence Data | - |
dc.title | The host-defence skin peptide profiles of Peron's Tree Frog Litoria peronii in winter and summer. Sequence determination by electrospray mass spectrometry and activities of the peptides | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/rcm.4164 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Musgrave, I. [0000-0003-1016-0588] | - |
Appears in Collections: | Aurora harvest 5 Chemistry publications |
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