Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/57213
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dc.contributor.author | Cooper, T. | - |
dc.contributor.author | McMurchie, E. | - |
dc.contributor.author | Leifert, W. | - |
dc.contributor.editor | Lundstrom, K. | - |
dc.contributor.editor | Chiu, M. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | G Protein-coupled receptors in drug discovery, 2009 / Lundstrom, K., Chiu, M. (ed./s), vol.552, pp.143-151 | - |
dc.identifier.isbn | 9781603273169 | - |
dc.identifier.uri | http://hdl.handle.net/2440/57213 | - |
dc.description.abstract | The [³⁵S]GTPγS binding assay to measure G protein activation following agonist binding to G protein-coupled receptors (GPCRs) remains a powerful molecular technique to substantiate traditional pharmacological values of potency, efficacy, and affinity. The method described uses membrane preparations of the α2A-adrenergic receptor and purified G protein subunits expressed in Sf9 cells, reconstituted into a functional signaling system. This technology is generic and could be used with other GPCRs to demonstrate initial signaling events following receptor activation. Agonist-stimulated [³⁵S]GTPγS binding is measured in a 96-well plate format using scintillation counting. | - |
dc.description.statementofresponsibility | Tamara Cooper, Edward J. McMurchie and Wayne R. Leifert | - |
dc.language.iso | en | - |
dc.publisher | Humana Press | - |
dc.relation.ispartofseries | Methods in Molecular Biology ; 552 | - |
dc.source.uri | http://dx.doi.org/10.1007/978-1-60327-317-6_10 | - |
dc.subject | [35S]GTPγS | - |
dc.subject | α2A-adrenergic receptor | - |
dc.subject | G protein activation | - |
dc.title | [³⁵S]GTPγS binding in G protein-coupled receptor assays | - |
dc.title.alternative | [(35)S]GTPgammaS binding in G protein-coupled receptor assays | - |
dc.type | Book chapter | - |
dc.identifier.doi | 10.1007/978-1-60327-317-6_10 | - |
dc.publisher.place | United States | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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