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https://hdl.handle.net/2440/59740
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Type: | Journal article |
Title: | Mitochondrial uncoupler FCCP activates proton conductance but does not block store-operated Ca2+ current in liver cells |
Author: | To, M. Aromataris, E. Castro, J. Roberts, M. Barritt, G. Rychkov, G. |
Citation: | Archives of Biochemistry and Biophysics, 2010; 495(2):152-158 |
Publisher: | Academic Press Inc |
Issue Date: | 2010 |
ISSN: | 0003-9861 1096-0384 |
Statement of Responsibility: | Minh-Son To, Edoardo C. Aromataris, Joel Castro, Michael L. Roberts, Greg J. Barritt and Grigori Y. Rychkov |
Abstract: | Uncouplers of mitochondrial oxidative phosphorylation, including carbonilcyanide p-triflouromethoxyphenylhydrazone (FCCP) and carbonilcyanide m-cholorophenylhydrazone (CCCP), are widely used in experimental research to investigate the role of mitochondria in cellular function. Unfortunately, it is very difficult to interpret the results obtained in intact cells using FCCP and CCCP, as these agents not only inhibit mitochondrial potential, but may also affect membrane potential and cell volume. Here we show by whole-cell patch clamping that in primary rat hepatocytes and H4IIE liver cells, FCCP induced large proton currents across the plasma membrane, but did not activate any other observable conductance. In intact hepatocytes FCCP inhibits thapsigargin-activated store-operated Ca(2+) entry, but in patch clamping under the conditions of strong Ca(2+) buffering it has no effect on store-operated Ca(2+) current (I(SOC)). These results indicate that there is no direct connection between mitochondria and activation of I(SOC) in liver cells and support the notion of indirect regulation of I(SOC) by mitochondrial Ca(2+) buffering. |
Keywords: | Liver Cells, Cultured Cell Line Mitochondria, Liver Hepatocytes Animals Rats Rats, Wistar Protons Calcium Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Fura-2 Uncoupling Agents Patch-Clamp Techniques Male |
Rights: | Copyright © 2010 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.abb.2010.01.004 |
Published version: | http://dx.doi.org/10.1016/j.abb.2010.01.004 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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