Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/60796
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Anti-inflammatory subtilase cytotoxin up-regulates A20 through the unfolded protein response |
Author: | Nakajima, S. Saito, Y. Takahashi, S. Hiramatsu, N. Kato, H. Johno, H. Yao, J. Paton, A. Paton, J. Kitamura, M. |
Citation: | Biochemical and Biophysical Research Communications, 2010; 397(2):176-180 |
Publisher: | Academic Press Inc |
Issue Date: | 2010 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Shotaro Nakajima, Yukinori Saito, Shuhei Takahashi, Nobuhiko Hiramatsu, Hironori Kato, Hisashi Johno, Jian Yao, Adrienne W. Paton, James C. Paton, Masanori Kitamura |
Abstract: | We recently reported that subtilase cytotoxin (SubAB) has the potential to attenuate experimental models of inflammatory diseases [3]. Currently, little is known about underlying mechanisms involved in this therapeutic effect. In the present report, we show that SubAB induces A20, the endogenous negative regulator of NF-kappaB, in vitro and in vivo. This stimulatory effect occurred at the transcriptional level, and SubAB induced activation of the A20 promoter. We found that, in the early phase, SubAB triggered activation of NF-kappaB in a dose-dependent manner. Blockade of NF-kappaB abrogated expression of A20 by SubAB. SubAB rapidly triggered the unfolded protein response (UPR), and induction of the UPR by other agents (thapsigargin and A23187) mimicked the stimulatory effects of SubAB, both on NF-kappaB and on A20. The induction of A20 by thapsigargin was correlated with activation of the A20 promoter, which was not observed in the kappaB-mutated A20 promoter. Furthermore, induction of A20 by SubAB was substantially attenuated by treatment with different chemical chaperones. These results elucidated for the first time that the anti-inflammatory SubAB has the potential to induce A20 through the UPR-NF-kappaB-dependent pathway. |
Keywords: | Subtilase cytotoxin A20 Unfolded protein response NF-κB |
Rights: | Copyright © 2010 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.bbrc.2010.05.069 |
Published version: | http://dx.doi.org/10.1016/j.bbrc.2010.05.069 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.