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https://hdl.handle.net/2440/62094
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Type: | Journal article |
Title: | Association of Helicobacter pylori with colorectal cancer development |
Author: | Alsamak, F. Abdulamir, A. Mahdi, L. Alnaib, K. Bakar, F. |
Citation: | Asian Biomedicine, 2010; 4(4):609-618 |
Publisher: | Chulalongkorn University |
Issue Date: | 2010 |
ISSN: | 1905-7415 1875-855X |
Statement of Responsibility: | Fadi Fouad Alsamak, Ahmed Sahib Abdulamir, Laila Khalid Mahdi, Khalid Alnaib and Fatimah Abu Bakar |
Abstract: | Background: Helicobacter pylori (H. pylori) may be associated with colorectal cancer. However, the underlying mechanisms are still unclear. Objectives: Explore the serostatus of H. pylori cytotoxicity-associated gene A product (CagA) in patients with colorectal carcinoma, and assess the association of H. pylori with colorectal cancer via c-Myc and MUC-2 proteins at tumor tissues. Methods: H. pylori CagA IgG antibodies were screened using enzyme-linked immunosorbent assay (ELISA) in 30 patients with colorectal carcinoma and 30 cancer-free control subjects. Paraffin-embedded blocks were examined for the expression of c-Myc and MUC-2 protein by immunohistochemistry. Results: H. pylori CagA seropositivity increased significantly among colorectal cancer patients (p <0.05). The expression of c-Myc and MUC-2 in colorectal carcinoma patients was over-expressed (80%), and downexpressed (63%) in resection margins (p <0.05). c-Myc over-expression and MUC-2 down-expression were associated with CagA-positive rather than CagA-negative H. pylori patients. In 16 CagA seropositive vs. 14 CagA seronegative patients, the expression rate was 97.3% vs. 64.2% and 33.3% vs. 78.5% for cMyc and MUC-2, respectively. CagA IgG level was significantly higher in positive than in negative c-Myc patients (p= 0.036), and in negative than in positive MUC-2 patients (p= 0.044). c-Myc and MUC-2 were positively and inversely correlated with CagA IgG level (p <0.05). Conclusions: CagA-seropositive H. pylori is most probably associated with colorectal cancer development. Part of the underlying mechanism for such association might be via alterations in expression of MUC-2, which depletes the mucous protective layer in the colo-rectum, and c-Myc, which stimulates the growth of cancerous cells. |
Keywords: | Helicobacter pylori colorectal cancer Cag-A MUC-2 |
Rights: | Copyright status unknown |
DOI: | 10.2478/abm-2010-0077 |
Description (link): | http://www.asianbiomed.org/AutoJournal.php |
Published version: | http://www.asianbiomed.org/htdocs/previous/A20104609.pdf |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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