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https://hdl.handle.net/2440/63056
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Type: | Journal article |
Title: | Whole-genome sequencing and gene mapping of a newly isolated lytic enterococcal bacteriophage EFRM31 |
Author: | Fard, R. Barton, M. Arthur, J. Heuzenroeder, M. |
Citation: | Archives of Virology, 2010; 2010(11):1887-1891 |
Publisher: | Springer Wien |
Issue Date: | 2010 |
ISSN: | 0304-8608 1432-8798 |
Statement of Responsibility: | Ramin Mazaheri Nezhad Fard, Mary D. Barton, Jane L. Arthur, Michael W. Heuzenroeder |
Abstract: | Bacteriophages contribute greatly to bacterial evolution. There has been limited investigation of enterococcal bacteriophages, and only two enterococcal bacteriophages have been sequenced completely. In this study, a novel enterococcal bacteriophage, EFRM31, was isolated from a piggery effluent sample and then characterized. The complete bacteriophage genome was determined by shotgun sequencing. EFRM31 belongs to the family Siphoviridae (order Caudovirales) and has a circular double-stranded DNA genome. The putative EFRM31 genome consists of 16945 nucleotides with a low GC content (34.5%) and does not contain CpG islands. The EFRM31 genome contains 82 putative open reading frames, including 17 with identities to genes required for the assembly of a head–tail bacteriophage and 6 hypothetical proteins of unknown function. In general, the sequencing results from EFRM31 revealed considerable similarity to another enterococcal bacteriophage, EFAP-1. This identity and the order of shared genes suggest a close relationship or a common ancestor for these two bacteriophages. |
Keywords: | Enterococcus Siphoviridae Genome, Viral Molecular Sequence Data |
Rights: | © Springer-Verlag 2010 |
DOI: | 10.1007/s00705-010-0800-3 |
Published version: | http://dx.doi.org/10.1007/s00705-010-0800-3 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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