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https://hdl.handle.net/2440/63249
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Type: | Conference paper |
Title: | Direct examination of cadmium bonding in rat tissues dosed with mine wastes and cadmium-containing solutions |
Author: | Diacomanolis, V. Ng, J. Sadler, R. Harris, H. Nomura, M. Noller, B. |
Citation: | AIP Conference Proceedings, 2010 / Garrett, R., Gentle, I., Nugent, K., Wilkins, S. (ed./s), vol.1234, pp.613-616 |
Publisher: | Springer New York LLC |
Publisher Place: | United States |
Issue Date: | 2010 |
Series/Report no.: | AIP Conference Proceedings |
ISBN: | 9780735407824 |
ISSN: | 0094-243X 1551-7616 |
Conference Name: | International Conference on Synchrotron Radiation and Instrumentation (27 Sep 2009 - 2 Oct 2009 : Melbourne, Australia) |
Editor: | Garrett, R. Gentle, I. Nugent, K. Wilkins, S. |
Statement of Responsibility: | V. Diacomanolis, J. C. Ng, R. Sadler, H. H. Harris, M. Nomura and B.N. Noller |
Abstract: | Direct examination by XANES and EXAFS of metal bonding in tissue can be demonstrated by examining cadmium uptake and bonding in animal tissue maintained at cryogenic temperatures. XANES at the K-edge of cadmium were collected at the Photon Factory Advanced Ring (PF-AR), NW10A beam line at KEK-Tsukuba-Japan. Rats fed with 1g mine waste containing 8-400 mg/kg cadmium per 200g body weight (b.w.) or dosed by oral gavage with either cadmium chloride solution alone (at 6 mg/kg b.w.) or in combination with other salts (As, Cu or Zn), 5 days/week for 6 weeks, had 0.1-7.5 and 8-86 mg/kg cadmium in the liver or kidney, respectively. Rats given intraperitoneally (ip) or intravenously (iv) 1-4 times with 1 mg/kg b.w. cadmium solution had 30-120 mg/kg cadmium in the liver or kidney. Tissues from rats were kept and transferred at cryogenic temperature and XANES were recorded at 20 K. The spectra for rat liver samples suggested conjugation of cadmium with glutathione or association with the sulfide bond (Cd-S) of proteins and peptides. EXAFS of rat liver fed by Cd and Zn solutions showed that Cd was clearly bound to S ligands with an inter-atomic distance of 2.54 Å for Cd-S that was similar to cadmium sulfide with an inter-atomic distance of 2.52 Å for Cd-S. Liver or kidney of rats fed with mine wastes did not give an edge in the XANES spectra indicating little uptake of cadmium by the animals. Longer and higher dosing regimen may be required in order to observe the same Cd-S bond in the rat tissue from mine wastes, including confirmation by EXAFS. © 2010 American Institute of Physics. |
Keywords: | XANES EXAFS cadmium bonding rat tissues mine waste |
Description: | Proceedings details: Proceedings of SRI 2009, 10th International Conference on Radiation Instrumentation |
Rights: | ©2010 American Institute of Physics |
DOI: | 10.1063/1.3463281 |
Grant ID: | http://purl.org/au-research/grants/arc/LP0214185 |
Published version: | http://dx.doi.org/10.1063/1.3463281 |
Appears in Collections: | Aurora harvest Chemistry publications Environment Institute publications |
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