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https://hdl.handle.net/2440/64433
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DC Field | Value | Language |
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dc.contributor.author | Haqshenas, G. | - |
dc.contributor.author | Dong, X. | - |
dc.contributor.author | Netter, H. | - |
dc.contributor.author | Torresi, J. | - |
dc.contributor.author | Gowans, E. | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Journal of General Virology, 2007; 88(3):895-902 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.issn | 1465-2099 | - |
dc.identifier.uri | http://hdl.handle.net/2440/64433 | - |
dc.description.abstract | Two GB virus B (GBV-B) chimeric genomes, GBV-HVR and GBV-HVRh (with a hinge), containing the coding region of the immunodominant hypervariable region 1 (HVR1) of the E2 envelope protein of Hepatitis C virus (HCV) were constructed. Immunoblot analysis confirmed that HVR1 was anchored to the GBV-B E2 protein. To investigate the replication competence and in vivo stability of in vitro-generated chimeric RNA transcripts, two naïve marmosets were inoculated intrahepatically with the transcripts. The GBV-HVR chimeric genome was detectable for 2 weeks post-inoculation (p.i.), whereas GBV-HVRh reverted to wild type 1 week p.i. Sequencing analysis of the HVR1 and flanking regions from GBV-HVR RNA isolated from marmoset serum demonstrated that the HVR1 insert remained unaltered in the GBV-HVR chimera for 2 weeks. Inoculation of a naïve marmoset with serum collected at 1 week p.i. also resulted in viraemia and confirmed that the serum contained infectious particles. All animals cleared the infection by 3 weeks p.i. and remained negative for the remaining weeks. The chimera may prove useful for the in vivo examination of any HCV HVR1-based vaccine candidates. | - |
dc.description.statementofresponsibility | G. Haqshenas, X. Dong, H. Netter, J. Torresi and E. J. Gowans | - |
dc.language.iso | en | - |
dc.publisher | Soc General Microbiology | - |
dc.rights | © 2007 SGM | - |
dc.source.uri | http://dx.doi.org/10.1099/vir.0.82467-0 | - |
dc.subject | Serum | - |
dc.subject | Animals | - |
dc.subject | Callithrix | - |
dc.subject | GB virus B | - |
dc.subject | Hepacivirus | - |
dc.subject | Viremia | - |
dc.subject | Flaviviridae Infections | - |
dc.subject | Viral Proteins | - |
dc.subject | RNA, Viral | - |
dc.subject | Immunoblotting | - |
dc.subject | Sequence Analysis, DNA | - |
dc.subject | Recombination, Genetic | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Base Sequence | - |
dc.subject | Genome, Viral | - |
dc.subject | Female | - |
dc.title | A chimeric GB virus B encoding the hepatitis C virus hypervariable region 1 is infectious in vivo | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1099/vir.0.82467-0 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Gowans, E. [0000-0002-4274-8311] | - |
Appears in Collections: | Aurora harvest Surgery publications |
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