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https://hdl.handle.net/2440/64611
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Type: | Journal article |
Title: | Comparative susceptibility of planktonic and 3-day-old Salmonella Typhimurium biofilms to disinfectants |
Author: | Wong, H. Townsend, K. Fenwick, S. Trengove, R. O'Handley, R. |
Citation: | Journal of Applied Microbiology, 2010; 108(6):2222-2228 |
Publisher: | Blackwell Publishing Ltd |
Issue Date: | 2010 |
ISSN: | 1364-5072 1365-2672 |
Statement of Responsibility: | H.S. Wong, K.M. Townsend, S.G. Fenwick, R.D. Trengove and R.M. O’Handley |
Abstract: | Aims: To compare the susceptibility of a 3-day-old biofilm and planktonic Salmonella to disinfectants at different exposure times. We hypothesize that Salmonella biofilms are more resilient to disinfectants compared to planktonic Salmonella. Methods and Results: The susceptibility of planktonic cells to disinfectants was tested by a modified version of the Council of Europe suspension test EN 1276. Salmonella biofilms were formed using the Calgary Biofilm Device. Results show that 3-day-old Salmonella biofilms are less susceptible to the disinfectants benzalkonium chloride, chlorhexidine gluconate, citric acid, quaternary ammonium compounds, sodium hypochlorite (SH) and ethanol, compared to planktonic Salmonella. Surprisingly, the results also demonstrate that low concentrations of SH were more effective against a 3-day-old biofilm compared to high concentrations of SH. Conclusions: While all the disinfectants evaluated were able to reduce biofilm-associated cells at concentrations and contact times sufficient to eliminate planktonic cells, there were still sufficient viable cells remaining in the biofilm to cause further contamination and potential infection. Significance and Impact of the Study: Protocols for the use of chemical disinfectants need to include biofilm susceptibility testing. There is a requirement for an effective and standardized tool for determining the susceptibility of biofilms to disinfectants. |
Keywords: | calgary biofilm device disinfectants planktonic Salmonella Typhimurium biofilm |
Rights: | © 2009 The Authors. Journal compilation © 2009 The Society for Applied Microbiology |
DOI: | 10.1111/j.1365-2672.2009.04630.x |
Published version: | http://dx.doi.org/10.1111/j.1365-2672.2009.04630.x |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
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