Adelaide Research & Scholarship
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https://hdl.handle.net/2440/65337
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| Type: | Journal article |
| Title: | Tyrosine phosphorylation enrichment and subsequent analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS |
| Author: | Condina, M. Klingler-Hoffmann, M. Hoffmann, P. |
| Citation: | Current protocols in protein science / editorial board, John E. Coligan ... [et al.], 2010; Suppl 2(13.11):1-26 |
| Publisher: | John wiley & Sons Inc |
| Issue Date: | 2010 |
| ISSN: | 1934-3663 1934-3663 |
| Statement of Responsibility: | Mark R. Condina, Manuela Klingler-Hoffmann and Peter Hoffmann |
| Abstract: | This unit describes methods to detect, identify, and characterize tyrosine phosphorylation in proteins by mass spectrometry, including sample preparation methods, enrichment strategies using phosphotyrosine-specific antibodies, and chromatographic separation methods. |
| Keywords: | Tyrosine Phosphotyrosine Proteins Antibodies, Phospho-Specific Chromatography, Liquid Peptide Mapping Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Protein Processing, Post-Translational Phosphorylation Chemistry Techniques, Analytical |
| Description: | Published Online: 1 NOV 2010 |
| Rights: | Copyright © 2010 by John Wiley & Sons, Inc. |
| DOI: | 10.1002/0471140864.ps1311s62 |
| Published version: | http://dx.doi.org/10.1002/0471140864.ps1311s62 |
| Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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