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https://hdl.handle.net/2440/66076
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dc.contributor.author | Fung, K. | - |
dc.contributor.author | Cursaro, V. | - |
dc.contributor.author | Lewanowitsch, T. | - |
dc.contributor.author | Brierley, G. | - |
dc.contributor.author | McColl, S. | - |
dc.contributor.author | Lockett, T. | - |
dc.contributor.author | Head, R. | - |
dc.contributor.author | Hoffmann, P. | - |
dc.contributor.author | Cosgrove, L. | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Proteomics, 2011; 11(5):964-971 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.issn | 1615-9861 | - |
dc.identifier.uri | http://hdl.handle.net/2440/66076 | - |
dc.description | Article first published online: 31 JAN 2011 | - |
dc.description.abstract | Many biologically active agents exert a pleiotropic response in cells and tissues. This presents challenges in descriptive and comparative analysis of the proteome in response to these agents. Although free-flow electrophoresis has been applied in a number of proteomic studies as a protein separation technique, the combination of free-flow electrophoresis and DIGE has not yet been investigated for comparative proteomic analysis. In this study, we have compared the effects of butyrate on HT29 colorectal cancer cells with a particular focus on apoptosis and describe the utility of a novel approach combining free-flow electrophoresis with DIGE to identify differentially expressed proteins. We verify the results obtained by the combined free-flow electrophoresis and DIGE approach with Western blot analysis of selected proteins. We also report for the first time the regulation of a number of proteins by butyrate in HT29 colorectal cells including peptidyl-prolyl cis-trans isomerase A (cyclophilin A) and profilin-1. | - |
dc.description.statementofresponsibility | Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Gemma V. Brierley, Shaun R. McColl, Trevor Lockett, Richard Head, Peter Hoffmann and Leah Cosgrove | - |
dc.language.iso | en | - |
dc.publisher | Wiley - V C H Verlag GmbH & Co. KGaA | - |
dc.rights | Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | - |
dc.source.uri | http://dx.doi.org/10.1002/pmic.201000429 | - |
dc.subject | Butyrate | - |
dc.subject | Colorectal cancer | - |
dc.subject | DIGE | - |
dc.subject | Free-flow electrophoresis | - |
dc.subject | Technology | - |
dc.title | A combined free-flow electrophoresis and DIGE approach to identify proteins regulated by butyrate in HT29 cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/pmic.201000429 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McColl, S. [0000-0003-0949-4660] | - |
dc.identifier.orcid | Head, R. [0000-0002-1196-0926] | - |
Appears in Collections: | Aurora harvest IPAS publications Molecular and Biomedical Science publications |
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