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https://hdl.handle.net/2440/66092
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Type: | Journal article |
Title: | The predominant role of IP₃ type 1 receptors in activation of store-operated Ca²⁺ entry in liver cells |
Other Titles: | The predominant role of IP(3) type 1 receptors in activation of store-operated Ca(2+) entry in liver cells |
Author: | Jones, L. Ma, L. Castro, J. Litjens, T. Barritt, G. Rychkov, G. |
Citation: | BBA: Biomembranes, 2011; 1808(3):745-751 |
Publisher: | Elsevier Science BV |
Issue Date: | 2011 |
ISSN: | 0005-2736 1878-2434 |
Statement of Responsibility: | L. Jones, L. Ma, J. Castro, T. Litjens, G.J. Barritt, G.Y. Rychkov |
Abstract: | Physiologically, hormone induced release of Ca²+ from intracellular stores occurs in response to inositol 1,4,5-trisphosphate (IP₃) binding to its receptors expressed on the membranes of intracellular organelles, mainly endoplasmic reticulum. These IP₃ receptors act as channels, releasing Ca²+ into the cytoplasmic space where it is responsible for regulating a host of distinct cellular processes. The depletion of intracellular Ca²+ stores leads to activation of store-operated Ca²+ channels on the plasma membrane which replenishes lost Ca²+ and sustain Ca²+ signalling. There are three isoforms of IP₃ receptor, each exhibiting distinctive properties, however, little is known about the role of each isoform in the activation of store-operated Ca²+ entry. Recent evidence suggest that at least in some cell types the endoplasmic reticulum is not a homogeneous Ca²+ store, and there might be a sub-compartment specifically linked to the activation of store-operated Ca²+ channels, and Ca²+ release activated Ca²+ (CRAC) channel in particular. Furthermore, this sub-compartment might express only certain types of IP₃ receptor but not the others. Here we show that H4IIE liver cells express all three types of IP₃ receptor, but only type 1 and to a lesser extent type 3, but not type 2, participate in the activation of CRAC current (I(CRAC)), while type 1 and type 2, but not type 3, participate in observed Ca²+ release in response to receptor stimulation. Presented results suggest that in H4IIE rat liver cells the sub-compartment of intracellular Ca²+ store linked to the activation of I(CRAC) predominantly expresses type 1 IP₃ receptors. |
Keywords: | CRAC channels IP₃ receptor Ca²⁺ stores |
Rights: | © 2010 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.bbamem.2010.12.013 |
Grant ID: | ARC NHMRC |
Published version: | http://dx.doi.org/10.1016/j.bbamem.2010.12.013 |
Appears in Collections: | Aurora harvest 5 Physiology publications |
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