Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/66431
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Type: | Journal article |
Title: | Transgenic zebrafish recapitulating tbx16 gene early developmental expression |
Author: | Wells, S. Nornes, S. Lardelli, M. |
Citation: | PLoS One, 2011; 6(6):e21559-1-e21559-6 |
Publisher: | Public Library of Science |
Issue Date: | 2011 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Escriva, H. |
Statement of Responsibility: | Simon Wells, Svanhild Nornes and Michael Lardelli |
Abstract: | We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons. |
Keywords: | Spinal Cord Neurons Interneurons Embryo, Nonmammalian Animals Animals, Genetically Modified Zebrafish T-Box Domain Proteins Zebrafish Proteins Green Fluorescent Proteins Organ Specificity Gene Expression Regulation, Developmental Genome Fluorescence |
Rights: | Copyright 2011 Wells et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0021559 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0021559 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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hdl_66431.pdf | Published version | 507.81 kB | Adobe PDF | View/Open |
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