Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/66450
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Comparison of characteristics and enzymatic products of leukocytes in the skin and laminar tissues of horses administered black walnut heartwood extract or lipopolysaccharide |
Author: | Riggs, L. Krunkosky, T. Noschka, E. Boozer, L. Moore, J. Robertson, T. |
Citation: | American Journal of Veterinary Research, 2009; 70(11):1383-1390 |
Publisher: | Amer Veterinary Medical Assoc |
Issue Date: | 2009 |
ISSN: | 0002-9645 1943-5681 |
Statement of Responsibility: | Laura M. Riggs, Thomas M. Krunkosky, Erik Noschka, Lindsay A. Boozer, James N. Moore, Thomas P. Robertson |
Abstract: | Objective—To compare characteristics and enzymatic products of leukocytes detected in the skin and laminar tissues of horses administered black walnut heartwood extract (BWHE) and horses administered purified lipopolysaccharide (LPS). Animals—25 healthy 5- to 15-year-old horses. Procedures—Horses were randomly assigned to receive LPS (20 ng of O55:B5 Escherichia coli endotoxin/kg; n = 5) IV or 6 L of BWHE (10) or water (control group; 10) via nasogastric intubation. Horses were euthanatized 12 hours after treatment or at onset of Obel grade 1 lameness. Laminar tissue samples and skin samples from the middle region of the neck were harvested at the time of euthanasia. Leukocyte emigration (determined via CD13 immunohistochemical analysis) and matrix metalloproteinase (MMP)-2 and MMP-9 gene expressions and activities (determined via reverse transcription PCR assay and gelatin zymography, respectively) were measured in skin and laminar tissue samples. Results—Tissues of horses receiving BWHE contained significantly higher numbers of CD13-positive cells and increased MMP-9 gene expression and activity, compared with findings in the other 2 groups. Values for laminar tissue and skin from LPS-treated horses were not increased, compared with findings in the control group, in any experiment. Conclusions and Clinical Relevance—Results indicated that BWHE administration causes increases in CD13-positive leukocyte numbers and MMP-9 expression and activity in laminar tissue and skin in horses; similar effects were not detected following LPS administration. Leukocyte emigration in horses with experimentally induced endotoxemia and in horses administered BWHE differed markedly, thereby providing additional evidence that the development of laminitis involves more complex mechanisms than endotoxemia-induced leukocyte activation alone. |
Keywords: | Leukocytes Hoof and Claw Skin Animals Horses Juglans Foot Diseases Horse Diseases Lipopolysaccharides Plant Extracts Wood Female Male |
Rights: | Copyright status unknown |
DOI: | 10.2460/ajvr.70.11.1383 |
Published version: | http://dx.doi.org/10.2460/ajvr.70.11.1383 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.