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https://hdl.handle.net/2440/6686
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Type: | Journal article |
Title: | Drug inhibition of the macrophage response to metal wear particles in vitro |
Author: | Haynes, D. Rogers, S. Howie, D. Pearcy, M. Vernon-Roberts, B. |
Citation: | Clinical Orthopaedics and Related Research, 1996; 323(323):316-326 |
Publisher: | LIPPINCOTT-RAVEN PUBL |
Issue Date: | 1996 |
ISSN: | 0009-921X 1528-1132 |
Statement of Responsibility: | David R. Haynes, Susan D. Rogers, Donald W. Howie, Mark J. Pearcy, and Barrie Vernon-Roberts |
Abstract: | The wear particles of cobalt chrome alloy and titanium alloy have been implicated as a cause of aseptic loosening of prostheses. It is thought that their ability to induce either cell death or the release of mediators that induce bone resorption contributes to this loosening. This study was designed to test the hypothesis that these adverse biologic effects are due to wear particle corrosion at low pH after they have been phagocytosed by macrophages. Cobalt chrome alloy and titanium alloy particles of similar size and concentration to those found in the tissues surrounding failed prostheses were added to cultured rodent peritoneal macrophages. Treatment of macrophages with drugs that prevent a drop in pH within phagosomes significantly reduced the toxicity of phagocytosed cobalt chrome alloy particles. The same drugs also reduced the levels of prostaglandin E2 and interleukin-6 release induced by phagocytosed titanium alloy particles. When both types of particles were incubated at a low pH, similar to that encountered by phagocytosed particles, soluble products were released that induced the same effects as the particles themselves. These results show that enhanced corrosion of wear particles by phagocytic cells may contribute significantly to the adverse biologic effects of wear particles and identify drug therapies that may be investigated further. |
Keywords: | Cells, Cultured Macrophages Animals Rats Ammonium Chloride Chromium Compounds Titanium Macrolides Piroxicam Cytochalasin B Chloroquine Dinoprostone Interleukin-6 Anti-Bacterial Agents Phagocytosis Corrosion Hydrogen-Ion Concentration |
Rights: | © 1996 Lippincott-Raven Publishers |
DOI: | 10.1097/00003086-199602000-00045 |
Published version: | http://dx.doi.org/10.1097/00003086-199602000-00045 |
Appears in Collections: | Aurora harvest Orthopaedics and Trauma publications |
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