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https://hdl.handle.net/2440/68549
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Type: | Journal article |
Title: | Uptake, distribution, and speciation of selenoamino acids by human cancer cells: X-ray absorption and fluorescence methods |
Author: | Weekley, C. Aitken, J. Vogt, S. Finney, L. Paterson, D. De Jonge, M. Howard, D. Musgrave, I. Harris, H. |
Citation: | Biochemistry, 2011; 50(10):1641-1650 |
Publisher: | Amer Chemical Soc |
Issue Date: | 2011 |
ISSN: | 0006-2960 1520-4995 |
Statement of Responsibility: | Claire M. Weekley, Jade B. Aitken, Stefan Vogt, Lydia A. Finney, David J. Paterson, Martin D. de Jonge, Daryl L. Howard, Ian F. Musgrave, and Hugh H. Harris |
Abstract: | Selenium compounds exhibit chemopreventative properties at supranutritional doses, but the efficacy of selenium supplementation in cancer prevention is dependent on the chemical speciation of the selenium supplement and its metabolites. The uptake, speciation, and distribution of the common selenoamino acid supplements, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys), in A549 human lung cancer cells were investigated using X-ray absorption and fluorescence spectroscopies. X-ray absorption spectroscopy of bulk cell pellets treated with the selenoamino acids for 24 h showed that while selenium was found exclusively in carbon-bound forms in SeMet-treated cells, a diselenide component was identified in MeSeCys-treated cells in addition to the carbon-bound selenium species. X-ray fluorescence microscopy of single cells showed that selenium accumulated with sulfur in the perinuclear region of SeMet-treated cells after 24 h, but microprobe selenium X-ray absorption near-edge spectroscopy in this region indicated that selenium was carbon-bound rather than sulfur-bound. X-ray absorption and X-ray fluorescence studies both showed that the selenium content of MeSeCys-treated cells was much lower than that of SeMet-treated cells. Selenium was distributed homogeneously throughout the MeSeCys-treated cells. |
Keywords: | Cell Line, Tumor Humans Neoplasms Organoselenium Compounds Selenocysteine Selenomethionine Cysteine Microscopy, Fluorescence X-Ray Absorption Spectroscopy |
Rights: | Copyright © 2011 American Chemical Society |
DOI: | 10.1021/bi101678a |
Grant ID: | http://purl.org/au-research/grants/arc/DP0984722 http://purl.org/au-research/grants/arc/DP0985807 http://purl.org/au-research/grants/arc/DP0984722 |
Published version: | http://dx.doi.org/10.1021/bi101678a |
Appears in Collections: | Aurora harvest Chemistry and Physics publications Environment Institute publications |
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