Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/68993
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Changes in cardiac troponins with gestational age explain changes in cardiac muscle contractility in the sheep fetus |
Author: | Posterino, Giuseppe Saverio Dunn, Stacey Leanne Botting, Kimberley Jae Wang, William Gentili, Sheridan Morrison, Janna Leigh |
Citation: | Journal of Applied Physiology, 2011; 111(1):236-243 |
Publisher: | Amer Physiological Soc |
Issue Date: | 2011 |
ISSN: | 8750-7587 |
School/Discipline: | School of Medical Sciences |
Statement of Responsibility: | Giuseppe Saverio Posterino, Stacey Leanne Dunn, Kimberley Jae Botting, William Wang, Sheridan Gentili and Janna Leigh Morrison |
Abstract: | The development of the adult cardiac troponin complex in conjunction with changes in cardiac function and cardiomyocyte binucleation has not been systematically characterized during fetal life in a species where maturation of the cardiomyocytes occurs prenatally as it does in the human. The aim of this study was to correlate the expression of each of the major adult troponin isoforms (T, I, and C) during late gestation (term of 150 days) to changes in both Ca²⁺ sensitivity and maximum Ca²⁺-activated force of the contractile apparatus and the maturation of cardiomyocytes. The percentage of mononucleated cardiomyocytes in the right ventricle decreased with gestational age to 46% by 137–142 days of gestation. The length of binucleated cardiomyocytes did not change with gestational age, but the length of binucleated cardiomyocytes relative to heart weight decreased with gestational age. There was no change in the expression of adult cardiac troponin T with increasing gestation. The contractile apparatus was significantly more sensitive to Ca²⁺ at 90 days compared with either 132 or 139 days of gestation, consistent with an ∼30% increase in the expression of adult cardiac troponin I between 90 and 110 days of gestation. Maximum Ca²⁺-activated force significantly increased from 90 days compared with 130 days consistent with an increase of ∼40% in cardiac troponin C protein expression. These data show that increased adult cardiac troponin I and C protein expression across late gestation is consistent with reduced Ca²⁺ sensitivity and increased maximum Ca²⁺-activated force. Furthermore, changes in cardiac troponin C, not I, protein expression track with the timing of cardiomyocyte binucleation. |
Keywords: | heart; calcium |
Rights: | Copyright © 2011 the American Physiological Society |
DOI: | 10.1152/japplphysiol.00067.2011 |
Appears in Collections: | Physiology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.