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https://hdl.handle.net/2440/7008
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dc.contributor.author | Hill, W. | - |
dc.contributor.author | Harper, G. | - |
dc.contributor.author | Rozaklis, T. | - |
dc.contributor.author | Hopwood, J. | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | Biochemical and molecular medicine, 1997; 62(1):85-94 | - |
dc.identifier.issn | 1077-3150 | - |
dc.identifier.issn | 1095-5577 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7008 | - |
dc.description.abstract | Cystic fibrosis is associated with mutations of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-regulated plasma membrane chloride channel. Cystic fibrosis patients have been reported to possess elevated sulfation of glycoconjugates, which may contribute to the pathogenesis of the disease. Sulfation of glycosaminoglycans by a cystic fibrosis pancreatic adenocarcinoma cell line homozygous for DeltaF508 (CFPAC-1), a control pancreatic cell line (PANC-1), two CFPAC-1 cell lines transfected with the gene for CFTR (PLJ-CFTR-4.7, TR20), and a mock-transfected CFPAC-1 control (PLJ-6) was investigated. Cells were radiolabeled with [35S]sulfate and [3H]glucosamine, and glycosaminoglycans secreted into the medium after 24 and 72 h were isolated. Chondroitinase ABC digestion of chondroitin/dermatan sulfate allowed the recovery of disaccharides which were analyzed for their degree of sulfation by strong anion-exchange HPLC. No differences in the extent of sulfation by any of the cell lines were noted. However, glycoaminoglycans synthesized by cystic fibrosis cells consistently exhibited twofold higher [35S]-sulfate:[3H]glucosamine ratios than the controls. We conclude that CFTR plays no role in the sulfation of chondroitin/dermatan sulfate by pancreatic cells and that isotope incorporation ratios alone are insufficient evidence of changes in sulfation levels. | - |
dc.description.statementofresponsibility | Warren G. Hill, Gregory S. Harper, Tina Rozaklis, John J. Hopwood | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.rights | Copyright © 1997, Elsevier | - |
dc.source.uri | http://dx.doi.org/10.1006/bmme.1997.2625 | - |
dc.subject | cystic fibrosis | - |
dc.subject | CFTR | - |
dc.subject | sulfation | - |
dc.subject | glycosaminoglycans | - |
dc.subject | pancreatic cells | - |
dc.title | Sulfation of chondroitin/dermatan sulfate by cystic fibrosis pancreatic duct cells is not different from control cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1006/bmme.1997.2625 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest Paediatrics publications |
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