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https://hdl.handle.net/2440/71019
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dc.contributor.author | Wang, T. | - |
dc.contributor.author | Tran, T. | - |
dc.contributor.author | Scanlon, D. | - |
dc.contributor.author | Andreazza, H. | - |
dc.contributor.author | Abell, A. | - |
dc.contributor.author | Bowie, J. | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Rapid Communications in Mass Spectrometry, 2011; 25(18):2649-2656 | - |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.issn | 1097-0231 | - |
dc.identifier.uri | http://hdl.handle.net/2440/71019 | - |
dc.description.abstract | It has been shown previously that [M-H](-) anions of small peptides containing two phosphate residues undergo cyclisation of the phosphate groups, following collision-induced dissociation (CID), to form a characteristic singly charged anion A (H3P2O7(-), m/z 177). In the present study it is shown that the precursor anions derived from the diphosphopeptides of caerin 1.1 [GLLSVLGSVAKHVLPHVVPVIAEHL(NH2)] and frenatin 3 [GLMSVLGHAVGNVLGGLFKPKS(OH)] also form the characteristic product anion A (m/z 177). Both of the precursor peptides show random structures in water, but partial helices in membrane-mimicking solvents [e.g. in d3-trifluoroethanol/water (1:1)]. In both cases the diphosphopeptide precursor anions must have flexible conformations in order to allow approach of the phosphate groups with consequent formation of A: for example, the two pSer groups of 4,22-diphosphofrenatin 3 are seventeen residues apart. Finally, CID tandem mass spectrometric (MS/MS) data from the [M-H](-) anion of the model triphosphoSer-containing peptide GpSGLGpSGLGpSGL(OH) show the presence of both product anions A (m/z 177) and D (m/z 257, H4P3O10(-)). Ab initio calculations at the HF/6-31+G(d)//AM1 level of theory suggest that cyclisation of the three phosphate groups occurs by a stepwise cascade mechanism in an energetically favourable reaction (ΔG = -245 kJ mol(-1)) with a maximum barrier of +123 kJ mol(-1). | - |
dc.description.statementofresponsibility | Tianfang Wang, T. T. Nha Tran, Denis Scanlon, Hayley J. Andreazza, Andrew D. Abell and John H. Bowie | - |
dc.language.iso | en | - |
dc.publisher | John Wiley & Sons Ltd | - |
dc.rights | Copyright © 2011 John Wiley & Sons, Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1002/rcm.5171 | - |
dc.subject | Polyphosphates | - |
dc.subject | Diphosphates | - |
dc.subject | Phosphopeptides | - |
dc.subject | Spectrometry, Mass, Electrospray Ionization | - |
dc.subject | Molecular Conformation | - |
dc.title | Diagnostic di- and triphosphate cyclisation in the negative ion electrospray mass spectra of phosphoSer peptides | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/rcm.5171 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Abell, A. [0000-0002-0604-2629] | - |
Appears in Collections: | Aurora harvest 5 IPAS publications |
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