Dot-blot enzyme immunoassay for the detection of bovine viral diarrhea virus antibodies

Abstract

Dot blot enzyme immunoassay (DB-EIA) was utilized for the detection of bovine viral diarrhea (BVD) antibodies in infected cattle. In this assay whole particles of the NADL strain of BVD virus were used as an antigen. A total of 1250 sera from SN and ELISA tested cows were tested for BVDV antibodies. HRPOconjugated protein G was used to detect bound BVDV antibodies using TMB as the substrate. Statistical analyses did not show any signififi cant differences in the sensitivity of DB-EIA, to the ELISA and SN tests. The assy proved to be a simple, inexpensive, reliable and rapid tool for BVD serodiagnosis.