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https://hdl.handle.net/2440/7223
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Type: | Journal article |
Title: | FcγRIIb expression on human germinal center B lymphocytes |
Other Titles: | Fc gammaRIIb expression on human germinal center B lymphocytes |
Author: | Macardle, P. Mardell, C. Bailey, S. Wheatland, L. Ho, A. Jessup, C. Roberton, D. Zola, H. |
Citation: | European Journal of Immunology, 2002; 32(12):3736-3744 |
Publisher: | Wiley-V C H Verlag GMBH |
Issue Date: | 2002 |
ISSN: | 0014-2980 1521-4141 |
Statement of Responsibility: | Peter J. Macardle, Carolyn Mardell, Sheree Bailey, Loretta Wheatland, Alice Ho, Claire Jessup, Donal M. Roberton and Heddy Zola |
Abstract: | IgG antibody can specifically suppress the antibody response to antigen. This has been explained by the hypothesis that signaling through the B cell antigen receptor is negatively modulated bythe co-ligation of immunoglobulin with the receptor for IgG, FcγRIIb. We hypothesized that inhibitory signaling through FcγRIIb would be counter-productive in germinal center cells undergoing selection by affinity maturation, since these cells are thought to receive a survival/proliferative signal by interacting with antigen displayed on follicular dendritic cells. We have identified and characterized a population of B lymphocytes with low/negative FcγRIIb expression that are present in human tonsil. Phenotypically these cells correspond to germinal center B cells and comprise both centroblast and centrocyte populations. In examining expression at the molecular level we determined that these B cells do not express detectable mRNA for FcγRIIb. We examined several culture conditions to induce expression of FcγRIIb on germinal center cells but could not determine conditions that altered expression. We then examined the functional consequence of cross-linking membrane immunoglobulin and the receptor for IgG on human B lymphocytes. Our results cast some doubt on the value of anti-IgG as a model for antigen–antibody complexes in studying human B cell regulation. |
Keywords: | Fc receptor B lymphocyte Lymph node Cellular differentiation |
Rights: | © 2002 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
DOI: | 10.1002/1521-4141(200212)32:12<3736::AID-IMMU3736>3.0.CO;2-I |
Published version: | http://dx.doi.org/10.1002/1521-4141(200212)32:12%3C3736::aid-immu3736%3E3.0.co;2-i |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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