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https://hdl.handle.net/2440/73083
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Type: | Journal article |
Title: | Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi |
Author: | van der Meer, M. Gardner, M. Hobbs, J. Jones, G. van Herwerden, L. |
Citation: | Conservation Genetics Resources, 2012; 4(2):247-250 |
Publisher: | Springer Netherlands |
Issue Date: | 2012 |
ISSN: | 1877-7252 1877-7260 |
Statement of Responsibility: | Martin H. van der Meer, Michael G. Gardner, Jean-Paul A. Hobbs, G. P. Jones, Lynne van Herwerden |
Abstract: | Endemic species at remote islands have a high risk of extinction because they often exhibit ecological, biological and genetic traits that make them particularly vulnerable to disturbances. McCulloch's anemonefish (Amphiprion mccullochi) is endemic to a few oceanic reefs off Australia's east coast and is an habitat specialist. Using 454 shotgun sequencing, we developed and evaluated primers for seventeen independent microsatellite loci to reveal gene flow, population genetic structure and genetic diversity across three isolated reefs. Observed and expected heterozygosities ranged from 0.556 to 1 and 0.543 to 0.907, respectively, in 30 Lord Howe Island individuals. When cross tested with the close relative, Amphiprion akindynos, amplification was successful with high levels of polymorphism. These loci will therefore be useful in studies of A. mccullochi, A. akindynos and possibly other closely related anemonefish. © 2011 Springer Science+Business Media B.V. |
Keywords: | Coral reef fish Island endemics Genetic diversity Cross-species amplification |
Rights: | © Springer Science+Business Media B.V. 2011 |
DOI: | 10.1007/s12686-011-9517-1 |
Published version: | http://dx.doi.org/10.1007/s12686-011-9517-1 |
Appears in Collections: | Aurora harvest Earth and Environmental Sciences publications Environment Institute publications |
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