Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/73513
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Type: Journal article
Title: Characterization of the wheat gene encoding a grain-specific lipid transfer protein TdPR61, and promoter activity in wheat, barley and rice
Author: Kovalchuk, N.
Gilson, J.
Bazanova, N.
Pyvovarenko, T.
Singh, R.
Shirley, N.
Ismagul, A.
Johnson, A.
Milligan, A.
Hrmova, M.
Langridge, P.
Lopato, S.
Citation: Journal of Experimental Botany, 2012; 63(5):2025-2040
Publisher: Oxford Univ Press
Issue Date: 2012
ISSN: 0022-0957
1460-2431
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Statement of
Responsibility: 
Nataliya Kovalchuk, Jessica Smith, Natalia Bazanova, Tatiana Pyvovarenko, Rohan Singh, Neil Shirley, Ainur Ismagul, Alexander Johnson, Andrew S. Milligan, Maria Hrmova, Peter Langridge and Sergiy Lopato
Abstract: The TaPR61 gene from bread wheat encodes a lipid transfer protein (LTP) with a hydrophobic signal peptide, predicted to direct the TaPR61 protein to the apoplast. Modelling of TaPR61 revealed the presence of an internal cavity which can accommodate at least two lipid molecules. The full-length gene, including the promoter sequence of a TaPR61 orthologue, was cloned from a BAC library of Triticum durum. Quantitative RT-PCR analysis revealed the presence of TaPR61 and TdPR61 mainly in grain. A transcriptional TdPR61 promoter-GUS fusion was stably transformed into wheat, barley, and rice. The strongest GUS expression in all three plants was found in the endosperm transfer cells, the embryo surrounding region (ESR), and in the embryo. The promoter is strong and has similar but not identical spatial patterns of activity in wheat, barley, and rice. These results suggest that the TdPR61 promoter will be a useful tool for improving grain quality by manipulating the quality and quantity of nutrient/lipid uptake to the endosperm and embryo. Mapping of regions important for the promoter function using transient expression assays in developing embryos resulted in the identification of two segments important for promoter activation in embryos. The putative cis-elements from the distal segment were used as bait in a yeast 1-hybrid (Y1H) screen of a cDNA library prepared from the liquid part of the wheat multinucleate syncytium. A transcription factor isolated in the screen is similar to BES1/BLZ1 from Arabidopsis, which is known to be a key transcriptional regulator of the brassinosteroid signalling pathway.
Keywords: Barley
BES1 transcription factor
embryo
endosperm transfer cells
lipid transfer protein
promoter
rice
wheat.
Rights: © The Author [2012].
DOI: 10.1093/jxb/err409
Grant ID: ARC
Published version: http://dx.doi.org/10.1093/jxb/err409
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest
Australian Centre for Plant Functional Genomics publications

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