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https://hdl.handle.net/2440/74158
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Type: | Journal article |
Title: | Backbone fragmentations of [M-H]⁻ anions from peptides. Reinvestigation of the mechanism of the beta prime cleavage |
Other Titles: | Backbone fragmentations of [M-H](-) anions from peptides. Reinvestigation of the mechanism of the beta prime cleavage |
Author: | Wang, T. Tran, T. Calabrese, A. Bowie, J. |
Citation: | Rapid Communications in Mass Spectrometry, 2012; 26(16):1832-1840 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2012 |
ISSN: | 0951-4198 1097-0231 |
Statement of Responsibility: | Tianfang Wang, T. T. Nha Tran, Antonio N. Calabrese, John H. Bowie |
Abstract: | <h4>Rationale</h4>An experimental study has shown that the structure of a β' ion proposed earlier is incorrect. Backbone cleavage β' anions have structures R(NH(-)) from systems [[RNHCH(X)CONHCH(Y)CO(2)H (or C-terminal CONH(2))-H](-) (where R is the rest of the peptide molecule and X and Y represent the α side chains of the individual amino acid residues).<h4>Methods</h4>Ab initio calculations were carried out at the CAM-B3LYP/6-311++g(d,p) level of theory.<h4>Conclusions</h4>The calculations suggest that RNH(-) ions are formed by S(N)i cyclisation processes involving either (i) the C-terminal CO(2)(-) or C-terminal [CONH](-) as appropriate, or (ii) an enolate ion [-NHC(-)(Y)-] cyclising at the backbone CH of the -CH(X)- group. Concomitant C-N bond cleavage then liberates an RNH(-) ion, processes which can occur along the peptide backbone. |
Keywords: | Anions Peptides Spectrometry, Mass, Electrospray Ionization Amino Acid Sequence Models, Molecular Molecular Sequence Data |
Rights: | Copyright © 2012 John Wiley & Sons, Ltd. |
DOI: | 10.1002/rcm.6297 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1002/rcm.6297 |
Appears in Collections: | Aurora harvest 4 Chemistry publications |
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