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https://hdl.handle.net/2440/7444
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DC Field | Value | Language |
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dc.contributor.author | Merry, C. | - |
dc.contributor.author | Lyon, M. | - |
dc.contributor.author | Deakin, J. | - |
dc.contributor.author | Hopwood, J. | - |
dc.contributor.author | Gallagher, J. | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of Biological Chemistry, 1999; 274(26):18455-18462 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | http://hdl.handle.net/2440/7444 | - |
dc.description.abstract | The heparan sulfates (HS) are hypervariable linear polysaccharides that act as membrane co-receptors for growth factors, chemokines, and extracellular matrix proteins. In most instances, the molecular basis of protein recognition by HS is poorly understood. We have sequenced 75% of the sulfated domains (S-domains) of fibroblast HS, including all of the major ones. This analysis revealed tight coupling of N- and 2-O-sulfation and a low frequency but precise positioning of 6-O-sulfates, which are required functional groups for HS-mediated activation of the fibroblast growth factors. S-domain sequencing was conducted using a novel and highly sensitive method based on a new way of reading the sequence from high performance liquid chromatography separation profiles of metabolically labeled HS-saccharides following specific chemical and enzymatic scission. The implications of the patterns seen in the sulfated domains for better understanding of the synthesis and function of HS are discussed. | - |
dc.description.statementofresponsibility | Catherine L. R. Merry, Malcolm Lyon, Jon A. Deakin, John J. Hopwood and John T. Gallagher | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.source.uri | http://dx.doi.org/10.1074/jbc.274.26.18455 | - |
dc.subject | 3T3 Cells | - |
dc.subject | Animals | - |
dc.subject | Mice | - |
dc.subject | Sulfates | - |
dc.subject | Heparitin Sulfate | - |
dc.subject | Chromatography, Gel | - |
dc.subject | Chromatography, High Pressure Liquid | - |
dc.subject | Carbohydrate Sequence | - |
dc.subject | Molecular Sequence Data | - |
dc.title | Highly sensitive sequencing of the sulfated domains of heparan sulfate | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1074/jbc.274.26.18455 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest Paediatrics publications |
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