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https://hdl.handle.net/2440/7463
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Type: | Journal article |
Title: | Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers |
Author: | Hawkes, J. Bryan, D. Gibson, R. |
Citation: | Journal of Clinical Immunology, 2002; 22(6):338-344 |
Publisher: | Kluwer Academic/plenum Publ |
Issue Date: | 2002 |
ISSN: | 0271-9142 1573-2592 |
Statement of Responsibility: | Joanna S. Hawkes, Dani-Louise Bryan and Robert A. Gibson |
Abstract: | Samples of milk (n=80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1β, interleukin-6, and tumor necrosis factor-α than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40–50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1β, interleukin-6, and tumor necrosis factor-α in the mammary gland and have the capacity to respond to further stimulation after leaving the breast. |
Keywords: | Human milk human milk cells peripheral blood mononuclear cells interleukin-1β interleukin-6 tumor necrosis factor-α |
Rights: | © Springer, Part of Springer Science+Business Media |
DOI: | 10.1023/A:1020652215048 |
Published version: | http://dx.doi.org/10.1023/a:1020652215048 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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