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https://hdl.handle.net/2440/74991
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Type: | Journal article |
Title: | Chemical synthesis of a fluorescent IGF-II analogue |
Author: | Cottam, J. Scanlon, D. Karas, J. Calabrese, A. Pukala, T. Forbes, B. Wallace, J. Abell, A. |
Citation: | International Journal of Peptide Research and Therapeutics, 2013; 19(1):61-69 |
Publisher: | Springer Netherlands |
Issue Date: | 2013 |
ISSN: | 1573-3149 1573-3904 |
Statement of Responsibility: | Jade M. Cottam, Denis B. Scanlon, John A. Karas, Antonio N. Calabrese, Tara L. Pukala, Briony E. Forbes, John C. Wallace, Andrew D. Abell |
Abstract: | Insulin-like growth factor II (IGF-II) is a protein with high structural and sequence similarity to insulin. Unlike insulin, it binds both the type 1 IGF receptor and the exon 11- isoform of the insulin receptor with high affinity. The overexpression and up regulation of IGF-II has been associated with the progression of various forms of cancer. The exact binding mechanism of IGF-II to its high affinity receptors is still not completely understood. Herein we describe the successful synthesis of a novel fluorescent IGF-II protein (F19Cou IGF-II), where residue 19 (phenylalanine) has been replaced by a fluorescent chromophore (coumaryl glycine). This novel coumaryl IGF-II analogue will be a useful tool for analysing the receptor interaction mechanisms in future studies. |
Keywords: | Insulin-like growth factor II Coumarin Solid phase peptide synthesis Pseudoproline Aggregation |
Rights: | © Springer Science+Business Media New York 2012 |
DOI: | 10.1007/s10989-012-9339-3 |
Published version: | http://dx.doi.org/10.1007/s10989-012-9339-3 |
Appears in Collections: | Aurora harvest IPAS publications |
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