Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/7504
Type: | Journal article |
Title: | Cutting edge: Human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP |
Author: | Tangye, S. Lazetic, S. Woollatt, E. Sutherland, G. Lanier, L. Phillips, J. |
Citation: | Journal of Immunology, 1999; 162(12):6981-6985 |
Issue Date: | 1999 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Stuart G. Tangye, Sasha Lazetic, Erica Woollatt, Grant R. Sutherland, Lewis L. Lanier, and Joseph H. Phillips |
Abstract: | The genetic defect in X-linked lymphoproliferative syndrome (XLP) is the Src homology 2 domain-containing protein SAP. SAP constitutively associates with the cell surface molecule, signaling lymphocytic activation molecule (SLAM), and competes with SH2-domain containing protein tyrosine phosphatase-2 (SHP-2) for recruitment to SLAM. SLAM exhibits homology with the mouse cell surface receptor 2B4. The human homologue of 2B4 has now been identified. It is recognized by the c1.7 mAb, a mAb capable of activating human NK cells. Human 2B4 became tyrosine phosphorylated following pervanadate-treatment of transfected cells and recruited SHP-2. SAP was also recruited to 2B4 in activated cells. Importantly, the 2B4-SAP interaction prevented the association between 2B4 and SHP-2. These results suggest that the phenotype of XLP may result from perturbed signaling not only through SLAM, but also other cell surface molecules that utilize SAP as a signaling adaptor protein. |
Keywords: | Killer Cells, Natural Cells, Cultured Humans Intracellular Signaling Peptides and Proteins Carrier Proteins Membrane Glycoproteins Receptors, Immunologic Antigens, CD Antibodies, Monoclonal Cloning, Molecular Signal Transduction Enzyme Activation Amino Acid Sequence src Homology Domains Phosphorylation Molecular Sequence Data Protein Tyrosine Phosphatases Protein Tyrosine Phosphatase, Non-Receptor Type 6 Protein Tyrosine Phosphatase, Non-Receptor Type 11 SH2 Domain-Containing Protein Tyrosine Phosphatases Protein Phosphatase 2 Signaling Lymphocytic Activation Molecule Family Signaling Lymphocytic Activation Molecule Associated Protein |
Published version: | http://www.jimmunol.org/content/162/12/6981 |
Appears in Collections: | Aurora harvest Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.