Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/76476
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii : Poeciliidae), an invasive species in Australia |
Author: | Umbers, K. Jennions, M. Gardner, M. Keogh, J. |
Citation: | Australian Journal of Zoology, 2012; 60(4):235-237 |
Publisher: | C S I R O Publishing |
Issue Date: | 2012 |
ISSN: | 0004-959X |
Statement of Responsibility: | Kate D. L. Umbers, Michael D. Jennions, Michael G. Gardner and J. Scott Keogh |
Abstract: | We isolated 25 new polymorphic microsatellite markers from the eastern mosquitofish, Gambusia holbrooki. Initially, 454 shotgun sequencing was used to identify 1187 loci for which primers could be designed. Of these 1187, we trialled 48 in the target species, 40 of which amplified a product of expected size. Subsequently, those 40 loci were screened for variation in 48 individuals from a single population in Canberra, Australia. Twenty loci were in Hardy–Weinberg equilibrium and polymorphic, with observed heterozygosity ranging from 0.04 to 0.72 (mean: 0.45 ± 0.18) and the number of alleles per locus ranged from 2 to 5 (mean: 3.20 ± 1.05). These loci will be useful in understanding genetic variation, paternity analysis and in managing this species across both its native and invasive range. |
Keywords: | 454 GS-FLX shotgun sequencing. |
Rights: | Journal compilation © CSIRO 2012 |
DOI: | 10.1071/ZO12095 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1071/zo12095 |
Appears in Collections: | Aurora harvest Earth and Environmental Sciences publications Environment Institute publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.