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https://hdl.handle.net/2440/7759
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dc.contributor.author | Callaghan, M. | - |
dc.contributor.author | Russell, A. | - |
dc.contributor.author | Woollatt, E. | - |
dc.contributor.author | Sutherland, G. | - |
dc.contributor.author | Sutherland, R. | - |
dc.contributor.author | Watts, C. | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | Oncogene, 1998; 17(26):3479-3491 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.issn | 1476-5594 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7759 | - |
dc.description.abstract | Use of the differential display technique to isolate progestin-regulated genes in T-47D human breast cancer cells led to identification of a novel gene, EDD. The cDNA sequence contains a 2799 amino acid open reading frame sharing 40% identity with the predicted 2894 amino acid product of the Drosophila melanogaster tumor suppressor gene hyperplastic discs, while the carboxy-terminal 889 amino acids show 96% identity to a rat 100 kDa HECT domain protein. EDD mRNA was progestin-induced in T-47D cells and was highly abundant in testes and expressed at moderately high levels in other tissues, suggesting a broad role for EDD. Anti-EDD antibodies immunoprecipitated an approximately 300 kDa protein from T-47D cell lysates. HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. EDD is likely to function as an E3 as in vitro translated protein bound ubiquitin reversibly through a conserved HECT domain cysteine residue. EDD was localized by FISH to chromosome 8q22, a locus disrupted in a variety of cancers. Given the homology between EDD and the hyperplastic discs protein, which is required for control of imaginal disc growth in Drosophila, EDD potentially has a role in regulation of cell proliferation or differentiation. | - |
dc.language.iso | en | - |
dc.publisher | STOCKTON PRESS | - |
dc.source.uri | http://dx.doi.org/10.1038/sj.onc.1202249 | - |
dc.subject | Chromosomes, Human, Pair 8 | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Rats | - |
dc.subject | Drosophila melanogaster | - |
dc.subject | Pregnenediones | - |
dc.subject | Ligases | - |
dc.subject | Peptide Synthases | - |
dc.subject | Ubiquitin-Protein Ligases | - |
dc.subject | Proteins | - |
dc.subject | Helminth Proteins | - |
dc.subject | Ubiquitins | - |
dc.subject | Blotting, Northern | - |
dc.subject | Genetic Techniques | - |
dc.subject | Cloning, Molecular | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Sequence Homology, Amino Acid | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Male | - |
dc.title | Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1038/sj.onc.1202249 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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